Background: Mechanisms driving the progression of chronic lymphocytic leukemia (CLL) from its early stages are not fully understood. The acquisition of molecular changes at the time of progression has been observed in a small fraction of patients, suggesting that CLL progression is not mainly driven by dynamic clonal evolution. In order to shed light on mechanisms that lead to CLL progression, we investigated longitudinal changes in both the genetic and immunological scenarios.

Methods: We performed genetic and immunological longitudinal analysis using paired primary samples from untreated CLL patients that underwent clinical progression (sampling at diagnosis and progression) and from patients with stable disease (sampling at diagnosis and at long-term asymptomatic follow-up).

Results: Molecular analysis showed limited and non-recurrent molecular changes at progression, indicating that clonal evolution is not the main driver of clinical progression. Our analysis of the immune kinetics found an increasingly dysfunctional CD8⁺ T cell compartment in progressing patients that was not observed in those patients that remained asymptomatic.

Specifically, terminally exhausted effector CD8⁺ T cells (T-bet^dim/-Eomes^hiPD1^hi) accumulated, while the the co-expression of inhibitory receptors (PD1, CD244 and CD160) increased, along with an altered gene expression profile in T cells only in those patients that progressed. In addition, malignant cells from patients at clinical progression showed enhanced capacity to induce exhaustion-related markers in CD8⁺ T cells ex vivo mainly through a mechanism dependent on soluble factors including IL-10.

Conclusions: Altogether, we demonstrate that the interaction with the immune microenvironment plays a key role in clinical progression in CLL, thereby providing a rationale for the use of early immunotherapeutic intervention.

 

Searching for signatures of positive selection in cytochrome b gene associated with subterranean lifestyle in fast-evolving arvicolines (Arvicolinae, Cricetidae, Rodentia)

Background: Mitochondrial genes encode proteins involved in oxidative phosphorylation. Variations in lifestyle and ecological niche can be directly reflected in metabolic performance. Subterranean rodents represent a good model for testing hypotheses on adaptive evolution driven by important ecological shifts.

 

Voles and lemmings of the subfamily Arvicolinae (Rodentia: Cricetidae) provide a good example for studies of adaptive radiation. This is the youngest group within the order Rodentia showing the fastest rates of diversification, including the transition to the subterranean lifestyle in several phylogenetically independent lineages.

Results: We evaluated the signatures of selection in the mitochondrial cytochrome b (cytB) gene in 62 Arvicolinae species characterized by either subterranean or surface-dwelling lifestyle by assessing amino acid sequence variation, exploring the functional consequences of the observed variation in the tertiary protein structure, and estimating selection pressure. Our analysis revealed that:

(1) three of the convergent amino acid substitutions were found among phylogenetically distant subterranean species and

(2) these substitutions may have an influence on the protein complex structure

(3) cytB showed an increased ω and evidence of relaxed selection in subterranean lineages, relative to non-subterranean, and

(4) eight protein domains possess increased nonsynonymous substitutions ratio in subterranean species.

Conclusions: Our study provides insights into the adaptive evolution of the cytochrome b gene in the Arvicolinae subfamily and its potential implications in the molecular mechanism of adaptation. We present a framework for future characterizations of the impact of specific mutations on the function, physiology, and interactions of the mtDNA-encoded proteins involved in oxidative phosphorylation.

Screening of wheat genotypes for the presence of common bunt resistance genes

Common bunt is known to cause grain yield and quality losses in wheat due to bunt ball formation and infestation of the grain. The aim of this study is to identify for sources of resistance to common bunt in wheat genotypes using phytopathological and molecular methods. In general, studied 60 Kazakh and foreign wheat genotypes were found 15 samples with the Bt9, Bt8 and Bt11 genes. Carriers of the Bt10 gene include the five varieties.

The four resistance genes, Bt8, Bt10, Bt11, Bt9, and Bt10 were identified in the Karasai variety. Phytopathological and molecular screening of Kazakh and foreign wheat genotypes selected 18 with genes for resistance to the disease. According to evaluation on an artificial infection 19 varieties showed an immune type of reaction.

These varieties will be used in breeding programs as donors to create resistant varieties against the common bunt. Thus, approaches can reduce the level of fungicides use and the most effective method to control the common bunt.

 

Genetic Mutations Associated with Pierre Robin Syndrome/Sequence: A Systematic Review

Pierre Robin syndrome/sequence (PRS) is associated with a triad of symptoms that includes micrognathia, cleft palate, and glossoptosis that may lead to respiratory obstruction. The syndrome occurs in 2 forms: nonsyndromic PRS (nsPRS), and PRS associated with other syndromes (sPRS). Studies have shown varying genetic mutations associated with both nsPRS and sPRS.

 

The present systematic review aims to provide a comprehensive collection of published literature reporting genetic mutations in PRS. Web of Science, PubMed, and Scopus were searched using the keywords: “Pierre Robin syndrome/sequence AND gene mutation.”

 

• The search resulted in 208 articles, of which 93 were excluded as they were duplicates/irrelevant.
• The full-text assessment led to the further exclusion of 76 articles. From the remaining 39 articles included in the review, details of 324 cases were extracted. 56% of the cases were sPRS, and 22% of the cases were associated with other malformations and the remaining were nsPRS.
• Genetic mutations were noted in 30.9% of the 300 cases. Based on the review, SOX9 was found to be the most common gene associated with both nsPRS and sPRS. The gene mutation in sPRS was specific to the associated syndrome.
• Due to the lack of original studies, a quantitative analysis was not possible. Thus, future studies must focus on conducting large-scale cohort studies.
• Along with generating data on genetic mutation, future studies must also conduct pedigree analysis to assess potential familial inheritance, which in turn could provide valuable insights into the etiopathogenesis of PRS.

 

HTLV-I p24 core recombinant antigen
00204-V-01mg	Virogen	0,1 mg	EUR 321
Description: HLTV-I p24 core recombinant antigen.

HTLV-I p24 core recombinant antigen
00204-V-1000ug	Virogen	1000 ug	EUR 1539
Description: HLTV-I p24 core recombinant antigen.

HTLV-I Envelope Recombinant Antigen
00203-V-01mg	Virogen	0,1 mg	EUR 321
Description: HLTV-I envelope recombinant antigen. C-terminus of gp-46 and most of p21.

HTLV-I Envelope Recombinant Antigen
00203-V-1000ug	Virogen	1000 ug	EUR 1539
Description: HLTV-I envelope recombinant antigen. C-terminus of gp-46 and most of p21.

HTLV-1 virus mosaic recombinant antigen
00205-V-01mg	Virogen	0,1 mg	EUR 321
Description: HTLV-1 virus mosaic recombinant antigen a.a. 374-400/190-207.

HTLV-1 virus mosaic recombinant antigen
00205-V-1000ug	Virogen	1000 ug	EUR 1539
Description: HTLV-1 virus mosaic recombinant antigen a.a. 374-400/190-207.

Recombinant HTLV-1 gag p19 Protein
VAng-Lsx7388-100g	Creative Biolabs	100 µg	EUR 2193.6
Description: HTLV type 1 gag p19, recombinant protein from yeast.

HTLV-1 virus mosaic recombinant antigen a.a. 374-400/190-207.
00205-V-100ugvial	Virogen	100 ug/vial	EUR 150
Description: HTLV-1 virus mosaic recombinant antigen a.a. 374-400/190-207.

Recombinant Human T-cell Lymphotropic Virus-I (HTLV-I), p24 Antigen
DAG503	Creative Diagnostics	1mg	EUR 1444.8

HTLV 1 ELISA Kit
VArk-Lsx012	Creative Biolabs	1 KIT	Ask for price
Description: Used for analyzing the presence of the human T-Cell lymphotropic viruses.

Rat HTLV-1 ELISA Kit
ERH0319	Abclonal	96Tests	EUR 625.2

Goat HTLV-1 ELISA Kit
EGTH0319	Abclonal	96Tests	EUR 625.2

Mouse HTLV-1 ELISA Kit
EMH0319	Abclonal	96Tests	EUR 625.2

Human HTLV-1 ELISA Kit
EHH0319	Abclonal	96Tests	EUR 625.2

Rabbit HTLV-1 ELISA Kit
ERTH0319	Abclonal	96Tests	EUR 625.2

Bovine HTLV-1 ELISA Kit
EBH0319	Abclonal	96Tests	EUR 625.2

Canine HTLV-1 ELISA Kit
ECH0319	Abclonal	96Tests	EUR 625.2

Porcine HTLV-1 ELISA Kit
EPH0319	Abclonal	96Tests	EUR 625.2

Anserine HTLV-1 ELISA Kit
EAH0319	Abclonal	96Tests	EUR 625.2

HTLV 1/2 ELISA Kit
VArk-Lsx011	Creative Biolabs	1 KIT	Ask for price
Description: Used for analyzing the presence of the human T-Cell lymphotropic viruses.

HTLV I p24 Protein
abx060600-1mg	Abbexa	1 mg	EUR 1779.6

HTLV-1 p24 Protein
20-abx260271	Abbexa	EUR 1062.00 EUR 410.40 EUR 1930.80	0.5 mg 100 ug 1 mg

Guinea Pig HTLV-1 ELISA Kit
EGH0319	Abclonal	96Tests	EUR 625.2

Recombinant HTLV-1 p24
7-07759	CHI Scientific	100µg	Ask for price

Recombinant HTLV-1 p24
7-07760	CHI Scientific	500µg	Ask for price

Recombinant HTLV-1 p24
7-07761	CHI Scientific	1000µg	Ask for price

Recombinant HTLV P24 Protein
VAng-Lsx0305-inquire	Creative Biolabs	inquire	Ask for price
Description: HTLV P24, recombinant protein from E. coli.

HTLV-1 p24 Core Protein
20-abx260143	Abbexa	EUR 1062.00 EUR 410.40 EUR 1646.40	0.5 mg 100 ug 1 mg

Recombinant HTLV-1 p24 Core
7-07756	CHI Scientific	100µg	Ask for price

Recombinant HTLV-1 p24 Core
7-07757	CHI Scientific	500µg	Ask for price

Recombinant HTLV-1 p24 Core
7-07758	CHI Scientific	1000µg	Ask for price

HTLV-1 TAX Antibody
20-abx137064	Abbexa	EUR 427.20 EUR 627.60	0.5 mg 1 mg

Human T-lymphotropicvirus?,HTLV-?ELISA Kit
201-12-1810	SunredBio	96 tests	EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Recombinant (E.Coli) HTLV-1 p24 Core
RP-540	Alpha Diagnostics	100 ug	EUR 343.2

Felis p27 core antigen ELISA Kit
ELA-E1491FELA-E	Lifescience Market	96 Tests	EUR 1113.6

Recombinant HTLV-1 P24 Protein [His]
VAng-Lsx0314-1mgEcoli	Creative Biolabs	1 mg (E. coli)	EUR 1126.8
Description: HTLV-1 P24 Protein [His], recombinant protein from E. coli.

Recombinant HTLV-1 P24 Core Protein
VAng-Lsx0313-100g	Creative Biolabs	100 µg	EUR 975.6
Description: HTLV type 1 P24 Core, recombinant protein from E. coli.

Recombinant HTLV-1 P24 Core Protein
VAng-Lsx0313-1mg	Creative Biolabs	1 mg	EUR 4104
Description: HTLV type 1 P24 Core, recombinant protein from E. coli.

Human T-Lymphotropic Virus (HTLV) ELISA Kit
abx055772-96tests	Abbexa	96 tests	EUR 801.6

Human HIV-1 And HIV-2 Plus P24 Antigen Fourth-Generation (HIV p24 antigen) ELISA Kit
abx257575-96tests	Abbexa	96 tests	EUR 764.4

Human T-lymphotropicvirusâ… /II(HTLV-â… /II) antibody ELISA kit
CSB-E14216h-24T	Cusabio	1 plate of 24 wells	EUR 198
Description: Qualitativeindirect ELISA kit for measuring Human T-lymphotropicvirusâ… /II (HTLV-â… /II) antibody in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human T-lymphotropicvirusâ… /II(HTLV-â… /II) antibody ELISA kit
1-CSB-E14216h	Cusabio	EUR 1080.00 EUR 6571.20 EUR 3480.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Qualitativeindirect ELISA kit for measuring Human T-lymphotropicvirusâ… /II(HTLV-â… /II) antibody in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human T-lymphotropicvirusⅠ(HTLV-Ⅰ)ELISA Kit
GA-E1826HM-48T	GenAsia Biotech	48T	EUR 346.8

Human T-lymphotropicvirusⅠ(HTLV-Ⅰ)ELISA Kit
GA-E1826HM-96T	GenAsia Biotech	96T	EUR 559.2

Human T-lymphotropicvirusⅠ(HTLV-Ⅰ)ELISA Kit
QY-E02392	Qayee Biotechnology	96T	EUR 433.2

Human T-Lymphotropic Virus 1 (HTLV-1) ELISA Kit
abx052972-96tests	Abbexa	96 tests	EUR 801.6

HIV Type 1 p24 Antigen ELISA (96 Determinations)
0801111	Zeptometrix	96T	EUR 621

HIV Type 1 p24 Antigen ELISA (96 Determinations)
801111	Zeptometrix	96 Determinations	EUR 591

HTLV-1 gp21 Protein
20-abx260284	Abbexa	EUR 1062.00 EUR 410.40 EUR 1930.80	0.5 mg 100 ug 1 mg

HTLV-1 gp46 Protein
20-abx260285	Abbexa	EUR 1062.00 EUR 410.40 EUR 1930.80	0.5 mg 100 ug 1 mg

Felis p27 core antigen,P27 ELISA Kit
CN-00273F1	ChemNorm	96T	EUR 523.2

Felis p27 core antigen,P28 ELISA Kit
CN-00273F2	ChemNorm	48T	EUR 344.4

HTLV-1 Mosaic Protein
20-abx260286	Abbexa	EUR 1062.00 EUR 410.40 EUR 1930.80	0.5 mg 100 ug 1 mg

HTLV type 1 Active p24 (aa 314 - 344)
DAG-P2586	Creative Diagnostics	200 µg	EUR 868.8

HIV Type 1 p24 Antigen ELISA 2.0 (96 Determinations)
801002	Zeptometrix	96 Determinations	EUR 574

HIV Type 1 p24 Antigen ELISA 2.0 (96 Determinations)
0801002	Zeptometrix	96T	EUR 603

HTLV-1 Envelope Protein
20-abx260142	Abbexa	EUR 1062.00 EUR 410.40 EUR 1646.40	0.5 mg 100 ug 1 mg

Recombinant HTLV-1 gp21
7-07765	CHI Scientific	100µg	Ask for price

Recombinant HTLV-1 gp21
7-07766	CHI Scientific	500µg	Ask for price

Recombinant HTLV-1 gp21
7-07767	CHI Scientific	1000µg	Ask for price

Recombinant HTLV-1 gp46
7-07768	CHI Scientific	100µg	Ask for price

Recombinant HTLV-1 gp46
7-07769	CHI Scientific	500µg	Ask for price

Recombinant HTLV-1 gp46
7-07770	CHI Scientific	1000µg	Ask for price

Recombinant HTLV-1 Mosaic
7-07771	CHI Scientific	100µg	Ask for price

Recombinant HTLV-1 Mosaic
7-07772	CHI Scientific	500µg	Ask for price

Recombinant HTLV-1 Mosaic
7-07773	CHI Scientific	1000µg	Ask for price

HTLV-1 Tax (11-19)
H-6096.0001	Bachem	1.0mg	EUR 138
Description: Sum Formula: C56H79N9O12; CAS# [141677-18-1] net

HTLV-1 Tax (11-19)
H-6096.0005	Bachem	5.0mg	EUR 441.6
Description: Sum Formula: C56H79N9O12; CAS# [141677-18-1] net

Recombinant HTLV-1 Protein
VAng-Lsx0308-inquire	Creative Biolabs	inquire	Ask for price
Description: HTLV type 1, recombinant protein from E. coli.

Recombinant HTLV-2 Protein
VAng-Lsx0309-inquire	Creative Biolabs	inquire	Ask for price
Description: HTLV type 2, recombinant protein from E. coli.

Recombinant HTLV-1 Envelope
7-07753	CHI Scientific	100µg	Ask for price

Recombinant HTLV-1 Envelope
7-07754	CHI Scientific	500µg	Ask for price

Recombinant HTLV-1 Envelope
7-07755	CHI Scientific	1000µg	Ask for price

HIV Type 1 p24 Antigen ELISA (5 X 96 Determinations)
0801200	Zeptometrix	5 X 96T	EUR 2563

HIV Type 1 p24 Antigen ELISA (5 X 96 Determinations)
801200	Zeptometrix	5 X 96 Determinations	EUR 2330

Dog S Antigen ELISA kit
E08S0054-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog S Antigen ELISA kit
E08S0054-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Canine S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog S Antigen ELISA kit
E08S0054-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Canine S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat S Antigen ELISA kit
E02S0054-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat S Antigen ELISA kit
E02S0054-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Rat S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat S Antigen ELISA kit
E02S0054-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Rat S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig S Antigen ELISA kit
E07S0054-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig S Antigen ELISA kit
E07S0054-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig S Antigen ELISA kit
E07S0054-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human T-Lymphotropic Virus 1+2 (HTLV-1/2) ELISA Kit
abx055384-96tests	Abbexa	96 tests	EUR 801.6

HIV Type 1 p24 Antigen ELISA 2.0 (5 X 96 Determinations)
801008	Zeptometrix	5 X 96 Determinations	EUR 2386

HIV Type 1 p24 Antigen ELISA 2.0 (5 X 96 Determinations)
0801008	Zeptometrix	5 X 96T	EUR 2505

HTLV-I Env. Protein
abx060597-1mg	Abbexa	1 mg	EUR 1612.8

HTLV I gp21 Protein
abx060598-100ug	Abbexa	100 ug	EUR 811.2