Background: Mechanisms driving the progression of chronic lymphocytic leukemia (CLL) from its early stages are not fully understood. The acquisition of molecular changes at the time of progression has been observed in a small fraction of patients, suggesting that CLL progression is not mainly driven by dynamic clonal evolution. In order to shed light on mechanisms that lead to CLL progression, we investigated longitudinal changes in both the genetic and immunological scenarios.
Methods: We performed genetic and immunological longitudinal analysis using paired primary samples from untreated CLL patients that underwent clinical progression (sampling at diagnosis and progression) and from patients with stable disease (sampling at diagnosis and at long-term asymptomatic follow-up).
Results: Molecular analysis showed limited and non-recurrent molecular changes at progression, indicating that clonal evolution is not the main driver of clinical progression. Our analysis of the immune kinetics found an increasingly dysfunctional CD8+ T cell compartment in progressing patients that was not observed in those patients that remained asymptomatic.
Specifically, terminally exhausted effector CD8+ T cells (T-betdim/-EomeshiPD1hi) accumulated, while the the co-expression of inhibitory receptors (PD1, CD244 and CD160) increased, along with an altered gene expression profile in T cells only in those patients that progressed. In addition, malignant cells from patients at clinical progression showed enhanced capacity to induce exhaustion-related markers in CD8+ T cells ex vivo mainly through a mechanism dependent on soluble factors including IL-10.
Conclusions: Altogether, we demonstrate that the interaction with the immune microenvironment plays a key role in clinical progression in CLL, thereby providing a rationale for the use of early immunotherapeutic intervention.
Searching for signatures of positive selection in cytochrome b gene associated with subterranean lifestyle in fast-evolving arvicolines (Arvicolinae, Cricetidae, Rodentia)
Background: Mitochondrial genes encode proteins involved in oxidative phosphorylation. Variations in lifestyle and ecological niche can be directly reflected in metabolic performance. Subterranean rodents represent a good model for testing hypotheses on adaptive evolution driven by important ecological shifts.
Voles and lemmings of the subfamily Arvicolinae (Rodentia: Cricetidae) provide a good example for studies of adaptive radiation. This is the youngest group within the order Rodentia showing the fastest rates of diversification, including the transition to the subterranean lifestyle in several phylogenetically independent lineages.
Results: We evaluated the signatures of selection in the mitochondrial cytochrome b (cytB) gene in 62 Arvicolinae species characterized by either subterranean or surface-dwelling lifestyle by assessing amino acid sequence variation, exploring the functional consequences of the observed variation in the tertiary protein structure, and estimating selection pressure. Our analysis revealed that:
(1) three of the convergent amino acid substitutions were found among phylogenetically distant subterranean species and
(2) these substitutions may have an influence on the protein complex structure
(3) cytB showed an increased ω and evidence of relaxed selection in subterranean lineages, relative to non-subterranean, and
(4) eight protein domains possess increased nonsynonymous substitutions ratio in subterranean species.
Conclusions: Our study provides insights into the adaptive evolution of the cytochrome b gene in the Arvicolinae subfamily and its potential implications in the molecular mechanism of adaptation. We present a framework for future characterizations of the impact of specific mutations on the function, physiology, and interactions of the mtDNA-encoded proteins involved in oxidative phosphorylation.

stjosephs-hospital
Screening of wheat genotypes for the presence of common bunt resistance genes
Common bunt is known to cause grain yield and quality losses in wheat due to bunt ball formation and infestation of the grain. The aim of this study is to identify for sources of resistance to common bunt in wheat genotypes using phytopathological and molecular methods. In general, studied 60 Kazakh and foreign wheat genotypes were found 15 samples with the Bt9, Bt8 and Bt11 genes. Carriers of the Bt10 gene include the five varieties.
The four resistance genes, Bt8, Bt10, Bt11, Bt9, and Bt10 were identified in the Karasai variety. Phytopathological and molecular screening of Kazakh and foreign wheat genotypes selected 18 with genes for resistance to the disease. According to evaluation on an artificial infection 19 varieties showed an immune type of reaction.
These varieties will be used in breeding programs as donors to create resistant varieties against the common bunt. Thus, approaches can reduce the level of fungicides use and the most effective method to control the common bunt.
Genetic Mutations Associated with Pierre Robin Syndrome/Sequence: A Systematic Review
Pierre Robin syndrome/sequence (PRS) is associated with a triad of symptoms that includes micrognathia, cleft palate, and glossoptosis that may lead to respiratory obstruction. The syndrome occurs in 2 forms: nonsyndromic PRS (nsPRS), and PRS associated with other syndromes (sPRS). Studies have shown varying genetic mutations associated with both nsPRS and sPRS.
The present systematic review aims to provide a comprehensive collection of published literature reporting genetic mutations in PRS. Web of Science, PubMed, and Scopus were searched using the keywords: “Pierre Robin syndrome/sequence AND gene mutation.”
- The search resulted in 208 articles, of which 93 were excluded as they were duplicates/irrelevant.
- The full-text assessment led to the further exclusion of 76 articles. From the remaining 39 articles included in the review, details of 324 cases were extracted. 56% of the cases were sPRS, and 22% of the cases were associated with other malformations and the remaining were nsPRS.
- Genetic mutations were noted in 30.9% of the 300 cases. Based on the review, SOX9 was found to be the most common gene associated with both nsPRS and sPRS. The gene mutation in sPRS was specific to the associated syndrome.
- Due to the lack of original studies, a quantitative analysis was not possible. Thus, future studies must focus on conducting large-scale cohort studies.
- Along with generating data on genetic mutation, future studies must also conduct pedigree analysis to assess potential familial inheritance, which in turn could provide valuable insights into the etiopathogenesis of PRS.
HTLV-I p24 core recombinant antigen |
00204-V-01mg |
Virogen |
0,1 mg |
EUR 321 |
|
Description: HLTV-I p24 core recombinant antigen. |
HTLV-I p24 core recombinant antigen |
00204-V-1000ug |
Virogen |
1000 ug |
EUR 1539 |
|
Description: HLTV-I p24 core recombinant antigen. |
HTLV-I Envelope Recombinant Antigen |
00203-V-01mg |
Virogen |
0,1 mg |
EUR 321 |
|
Description: HLTV-I envelope recombinant antigen. C-terminus of gp-46 and most of p21. |
HTLV-I Envelope Recombinant Antigen |
00203-V-1000ug |
Virogen |
1000 ug |
EUR 1539 |
|
Description: HLTV-I envelope recombinant antigen. C-terminus of gp-46 and most of p21. |
HTLV-1 virus mosaic recombinant antigen |
00205-V-01mg |
Virogen |
0,1 mg |
EUR 321 |
|
Description: HTLV-1 virus mosaic recombinant antigen a.a. 374-400/190-207. |
HTLV-1 virus mosaic recombinant antigen |
00205-V-1000ug |
Virogen |
1000 ug |
EUR 1539 |
|
Description: HTLV-1 virus mosaic recombinant antigen a.a. 374-400/190-207. |
Recombinant HTLV-1 gag p19 Protein |
VAng-Lsx7388-100g |
Creative Biolabs |
100 µg |
EUR 2193.6 |
Description: HTLV type 1 gag p19, recombinant protein from yeast. |
HTLV-1 virus mosaic recombinant antigen a.a. 374-400/190-207. |
00205-V-100ugvial |
Virogen |
100 ug/vial |
EUR 150 |
|
Description: HTLV-1 virus mosaic recombinant antigen a.a. 374-400/190-207. |
Recombinant Human T-cell Lymphotropic Virus-I (HTLV-I), p24 Antigen |
DAG503 |
Creative Diagnostics |
1mg |
EUR 1444.8 |
HTLV 1 ELISA Kit |
VArk-Lsx012 |
Creative Biolabs |
1 KIT |
Ask for price |
Description: Used for analyzing the presence of the human T-Cell lymphotropic viruses. |
Rat HTLV-1 ELISA Kit |
ERH0319 |
Abclonal |
96Tests |
EUR 625.2 |
Goat HTLV-1 ELISA Kit |
EGTH0319 |
Abclonal |
96Tests |
EUR 625.2 |
Mouse HTLV-1 ELISA Kit |
EMH0319 |
Abclonal |
96Tests |
EUR 625.2 |
Human HTLV-1 ELISA Kit |
EHH0319 |
Abclonal |
96Tests |
EUR 625.2 |
Rabbit HTLV-1 ELISA Kit |
ERTH0319 |
Abclonal |
96Tests |
EUR 625.2 |
Bovine HTLV-1 ELISA Kit |
EBH0319 |
Abclonal |
96Tests |
EUR 625.2 |
Canine HTLV-1 ELISA Kit |
ECH0319 |
Abclonal |
96Tests |
EUR 625.2 |
Porcine HTLV-1 ELISA Kit |
EPH0319 |
Abclonal |
96Tests |
EUR 625.2 |
Anserine HTLV-1 ELISA Kit |
EAH0319 |
Abclonal |
96Tests |
EUR 625.2 |
HTLV 1/2 ELISA Kit |
VArk-Lsx011 |
Creative Biolabs |
1 KIT |
Ask for price |
Description: Used for analyzing the presence of the human T-Cell lymphotropic viruses. |
HTLV I p24 Protein |
abx060600-1mg |
Abbexa |
1 mg |
EUR 1779.6 |
|
HTLV-1 p24 Protein |
20-abx260271 |
Abbexa |
-
EUR 1062.00
-
EUR 410.40
-
EUR 1930.80
|
|
|
Guinea Pig HTLV-1 ELISA Kit |
EGH0319 |
Abclonal |
96Tests |
EUR 625.2 |
Recombinant HTLV P24 Protein |
VAng-Lsx0305-inquire |
Creative Biolabs |
inquire |
Ask for price |
Description: HTLV P24, recombinant protein from E. coli. |
HTLV-1 p24 Core Protein |
20-abx260143 |
Abbexa |
-
EUR 1062.00
-
EUR 410.40
-
EUR 1646.40
|
|
|
Recombinant HTLV-1 p24 Core |
7-07756 |
CHI Scientific |
100µg |
Ask for price |
Recombinant HTLV-1 p24 Core |
7-07757 |
CHI Scientific |
500µg |
Ask for price |
Recombinant HTLV-1 p24 Core |
7-07758 |
CHI Scientific |
1000µg |
Ask for price |
HTLV-1 TAX Antibody |
20-abx137064 |
Abbexa |
|
|
|
Human T-lymphotropicvirus?,HTLV-?ELISA Kit |
201-12-1810 |
SunredBio |
96 tests |
EUR 528 |
|
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Recombinant HTLV-1 P24 Protein [His] |
VAng-Lsx0314-1mgEcoli |
Creative Biolabs |
1 mg (E. coli) |
EUR 1126.8 |
Description: HTLV-1 P24 Protein [His], recombinant protein from E. coli. |
Recombinant HTLV-1 P24 Core Protein |
VAng-Lsx0313-100g |
Creative Biolabs |
100 µg |
EUR 975.6 |
Description: HTLV type 1 P24 Core, recombinant protein from E. coli. |
Recombinant HTLV-1 P24 Core Protein |
VAng-Lsx0313-1mg |
Creative Biolabs |
1 mg |
EUR 4104 |
Description: HTLV type 1 P24 Core, recombinant protein from E. coli. |
Human T-Lymphotropic Virus (HTLV) ELISA Kit |
abx055772-96tests |
Abbexa |
96 tests |
EUR 801.6 |
|
Human HIV-1 And HIV-2 Plus P24 Antigen Fourth-Generation (HIV p24 antigen) ELISA Kit |
abx257575-96tests |
Abbexa |
96 tests |
EUR 764.4 |
|
Human T-lymphotropicvirusâ… /II(HTLV-â… /II) antibody ELISA kit |
CSB-E14216h-24T |
Cusabio |
1 plate of 24 wells |
EUR 198 |
|
Description: Qualitativeindirect ELISA kit for measuring Human T-lymphotropicvirusâ… /II (HTLV-â… /II) antibody in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Human T-lymphotropicvirusâ… /II(HTLV-â… /II) antibody ELISA kit |
1-CSB-E14216h |
Cusabio |
-
EUR 1080.00
-
EUR 6571.20
-
EUR 3480.00
|
-
1 plate of 96 wells
-
10 plates of 96 wells each
-
5 plates of 96 wells each
|
|
Description: Qualitativeindirect ELISA kit for measuring Human T-lymphotropicvirusâ… /II(HTLV-â… /II) antibody in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Human T-lymphotropicvirusⅠ(HTLV-Ⅰ)ELISA Kit |
GA-E1826HM-48T |
GenAsia Biotech |
48T |
EUR 346.8 |
Human T-lymphotropicvirusⅠ(HTLV-Ⅰ)ELISA Kit |
GA-E1826HM-96T |
GenAsia Biotech |
96T |
EUR 559.2 |
Human T-Lymphotropic Virus 1 (HTLV-1) ELISA Kit |
abx052972-96tests |
Abbexa |
96 tests |
EUR 801.6 |
|
HIV Type 1 p24 Antigen ELISA (96 Determinations) |
0801111 |
Zeptometrix |
96T |
EUR 621 |
HIV Type 1 p24 Antigen ELISA (96 Determinations) |
801111 |
Zeptometrix |
96 Determinations |
EUR 591 |
HTLV-1 gp21 Protein |
20-abx260284 |
Abbexa |
-
EUR 1062.00
-
EUR 410.40
-
EUR 1930.80
|
|
|
HTLV-1 gp46 Protein |
20-abx260285 |
Abbexa |
-
EUR 1062.00
-
EUR 410.40
-
EUR 1930.80
|
|
|
Felis p27 core antigen,P27 ELISA Kit |
CN-00273F1 |
ChemNorm |
96T |
EUR 523.2 |
Felis p27 core antigen,P28 ELISA Kit |
CN-00273F2 |
ChemNorm |
48T |
EUR 344.4 |
HTLV-1 Mosaic Protein |
20-abx260286 |
Abbexa |
-
EUR 1062.00
-
EUR 410.40
-
EUR 1930.80
|
|
|
HIV Type 1 p24 Antigen ELISA 2.0 (96 Determinations) |
801002 |
Zeptometrix |
96 Determinations |
EUR 574 |
HIV Type 1 p24 Antigen ELISA 2.0 (96 Determinations) |
0801002 |
Zeptometrix |
96T |
EUR 603 |
HTLV-1 Envelope Protein |
20-abx260142 |
Abbexa |
-
EUR 1062.00
-
EUR 410.40
-
EUR 1646.40
|
|
|
HTLV-1 Tax (11-19) |
H-6096.0001 |
Bachem |
1.0mg |
EUR 138 |
Description: Sum Formula: C56H79N9O12; CAS# [141677-18-1] net |
HTLV-1 Tax (11-19) |
H-6096.0005 |
Bachem |
5.0mg |
EUR 441.6 |
Description: Sum Formula: C56H79N9O12; CAS# [141677-18-1] net |
Recombinant HTLV-1 Protein |
VAng-Lsx0308-inquire |
Creative Biolabs |
inquire |
Ask for price |
Description: HTLV type 1, recombinant protein from E. coli. |
Recombinant HTLV-2 Protein |
VAng-Lsx0309-inquire |
Creative Biolabs |
inquire |
Ask for price |
Description: HTLV type 2, recombinant protein from E. coli. |
Recombinant HTLV-1 Envelope |
7-07753 |
CHI Scientific |
100µg |
Ask for price |
Recombinant HTLV-1 Envelope |
7-07754 |
CHI Scientific |
500µg |
Ask for price |
Recombinant HTLV-1 Envelope |
7-07755 |
CHI Scientific |
1000µg |
Ask for price |
HIV Type 1 p24 Antigen ELISA (5 X 96 Determinations) |
0801200 |
Zeptometrix |
5 X 96T |
EUR 2563 |
HIV Type 1 p24 Antigen ELISA (5 X 96 Determinations) |
801200 |
Zeptometrix |
5 X 96 Determinations |
EUR 2330 |
Dog S Antigen ELISA kit |
E08S0054-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Canine S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog S Antigen ELISA kit |
E08S0054-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Canine S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog S Antigen ELISA kit |
E08S0054-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Canine S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat S Antigen ELISA kit |
E02S0054-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Rat S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat S Antigen ELISA kit |
E02S0054-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Rat S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat S Antigen ELISA kit |
E02S0054-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Rat S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig S Antigen ELISA kit |
E07S0054-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Porcine S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig S Antigen ELISA kit |
E07S0054-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Porcine S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig S Antigen ELISA kit |
E07S0054-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Porcine S Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human T-Lymphotropic Virus 1+2 (HTLV-1/2) ELISA Kit |
abx055384-96tests |
Abbexa |
96 tests |
EUR 801.6 |
|
HIV Type 1 p24 Antigen ELISA 2.0 (5 X 96 Determinations) |
801008 |
Zeptometrix |
5 X 96 Determinations |
EUR 2386 |
HIV Type 1 p24 Antigen ELISA 2.0 (5 X 96 Determinations) |
0801008 |
Zeptometrix |
5 X 96T |
EUR 2505 |
HTLV-I Env. Protein |
abx060597-1mg |
Abbexa |
1 mg |
EUR 1612.8 |
|
HTLV I gp21 Protein |
abx060598-100ug |
Abbexa |
100 ug |
EUR 811.2 |
|