Our Lightning-Hyperlink HRP antibody labeling gear permits the direct conjugation of your antibody, protein, or peptide to HRP for use in any software program. The gear solely requires 30 seconds of hands-on time, and conjugates are ready to utilize after solely three hours. Furthermore, there is not a need for added wash or separation steps submit conjugation.
Suggestions for Use of ab171537 HRP Stability Conjugate Reagent
- For optimum stability and effectivity, use ab171537 at 100% focus
- Dilute your HRP conjugate to optimum working focus inside the HRP Stability Conjugate Reagent decision
- Use diluted conjugate working decision in your assay as common
- Or, ideally, retailer away from direct gentle at room temperature and use as a stock decision for optimum assay-to-assay consistency
PROPERTIES
producer/tradename
Upstate
software program(s)
ELISA: acceptable
dot blot: acceptable
immunohistochemistry: acceptable
western blot: acceptable
shipped in
moist ice
DESCRIPTION
Software program
Bodily type
Storage and Stability
Licensed Knowledge
Disclaimer
HRP Conjugate Reagent
NOTE – When storing any conjugate/ HRP Stability Conjugate Reagent decision for an extended time interval: If a phosphate buffer is used for dilution we recommend a remaining focus of no more than 5 mM buffer to avoid the potential of initiating precipitation.
Immediately label your main antibody with HRP
Horseradish peroxidase (HRP) is a 44 kDa glycoprotein with 6 lysine residues, which may very well be conjugated to antibodies and proteins for use in a variety of functions.
The enzyme label may very well be visualized through chromogenic reactions. For example, diaminobenzidine (DAB) inside the presence of hydrogen peroxide (H202) is reworked proper right into a water-insoluble brown pigment. Completely different substrates that may be utilized to measure horseradish peroxidase train embrace ABTS, TMB, and TMBUS.
HRP is a popular detection label utilized in evaluation. Antibody-HRP conjugates are typically utilized in ELISA, IHC, and western blotting. HRP may very well be conjugated to the primary antibody for direct detection or secondary antibody for indirect detection.
Direct detection is normally a preferred methodology inside these functions to avoid cross-species reactivity and to eradicate additional laborious wash and separation steps, notably in time-consuming protocols. Nonetheless, immediately conjugating HRP to the antibody or protein of choice may very well be powerful and labor-intensive if using typical methodology.
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Description: Gli1 Antibody detects endogenous levels of Gli1. |
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Description: A polyclonal antibody against GLI1. Recognizes GLI1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:200-1:500, IF:1:50-1:200 |
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Description: This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. |
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Description: This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. |
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| C43926 | SAB | 100ul | EUR 397 |
