qPCR and One-Step RT MasterMixes

Viral RNA/DNA titer using qRT-PCR.

Virus Titration Kits

Our Titration Kits current a fast and straightforward approach for titrating your virus. The kits make use of each a quick RNA or DNA extraction step, and resolve viral RNA/DNA titer using qRT-PCR.

Advantages of abm’s titration kits:

  • No RNA purification — saves time and eliminates inaccuracy.
  • Ready-to-use reagent mix — reduces variability.
  • qRT-PCR in a single step — additional delicate and proper than completely different methods.
  • No NTC amplification — Distinctive primer design completely eliminates non-specific amplification in NTCs.

We moreover provide On the spot Lentivirus Titration Taking part in playing cards, which allow quick detection of lentiviral capsule proteins as a marker to substantiate the presence of lentivirus. Utilizing the lateral flow into immunoassay approach, viral titer yields are assessed instantly inside 10 minutes using our three step protocol.

he Retro-X qRT-PCR Titration Tools provides a fast and straightforward approach for measuring retrovirus titer. The kits use a quick RNA purification step sooner than quantifying the number of retroviral genome copies using qRT-PCR and TB Inexperienced utilized sciences.

qPCR and One-Step RT MasterMixes

qPCR and One-Step RT MasterMixes

Description

abm’s qPCR Retrovirus Titer Tools is a one-step assay by qPCR which does not require additional lysis or reverse transcription (RT) steps, and might be completed beneath one hour. Designed to ship extreme sensitivity and specificity, the tools ensures minimal non-specific background and better complete effectivity as compared with comparable kits within the market. ROX reference dye is obtainable separate from the MasterMix, making this tools universally appropriate with most qPCR gadgets.

PRODUCT COMPONENT QUANTITY
BlasTaq 2X qPCR Titer MasterMix 1.25 ml
Primer Mix 100 rxn (200 µl)
Commonplace Administration DNA 50 µl
ROX Reference Dye 15 µl
Nuclease-Free H2O 2 x 1.zero ml

 

SKU G949
Unit quantity 100 rxn
Storage State of affairs Retailer at -20°C

 

Quick willpower of retroviral titer

Whereas standard titration methods require as a lot as 10 days to complete, these titration kits require solely 4 hours and work with all MMLV-based retroviral vectors (not MSCV). Using qRT-PCR dramatically shortens the time interval between viral harvest and target-cell an an infection, allowing you to hold out every on the equivalent day.

Receive fixed retroviral transductions

A critical good thing about retroviral gene-delivery strategies is the facility to manage the extent of gene expression by adjusting the multiplicity of an an infection (MOI) to dictate the number of viral genomes that enter your objective cells. These titration kits allow you to make a quick correlation between the retroviral copy amount and retroviral infectivity in your cell type and in the long run allow you to administration the expression stage between experiments.

The best way it really works

The qRT-PCR course of entails subjecting serial dilutions of your sample and a administration RNA of acknowledged copy amount (provided) to one-step qRT-PCR. The retroviral copy amount contained in your sample can then be determined by evaluating its Ct value to the standard curve.

RNA purification and DNase I remedy steps are essential to the protocol so even viruses packaged using transient transfections, which always embrace residual plasmid DNA, might be exactly quantified using this tools.

 

Retrovirus 10x Titer-Up Kit Test Sample (0.1 mL)
P909S - Ask for price

Retrovirus Mini Purification Kit
K1307-10 each
EUR 652.8

Retrovirus Mini Purification Kit
K1307-20 each
EUR 1096.8

Retrovirus Maxi Purification Kit
K1308-10 each
EUR 1554

Retrovirus Maxi Purification Kit
K1308-2 each
EUR 639.6

Retrovirus Maxi Purification Kit
K1308-4 each
EUR 1032

Retrovirus Booster 50µl
ABP-RVC-RBST05 50µl Ask for price

Retrovirus Booster 200µl
ABP-RVC-RBST20 200µl Ask for price

Retrovirus Booster 400µl
ABP-RVC-RBST40 400µl Ask for price

Retrovirus iPSC Reprogramming Kit
RF101 1 Kit
EUR 782.43

Retrovirus-related Gag
E8R1706-5 100ul
EUR 275
Description: Available in various conjugation types.

Retrovirus Stabilizer 50µl
ABP-RVC-RSTB05 50µl Ask for price

Retrovirus Stabilizer 200µl
ABP-RVC-RSTB20 200µl Ask for price

Retrovirus Stabilizer 400µl
ABP-RVC-RSTB40 400µl Ask for price

iPS Cell Line (Retrovirus)
ASE-9101 1 vial of ^2.5X10^5 cells
EUR 516.84
Description: 6 month

M13 Phage Titration ELISA Kit
55R-PRPHAGE 96 tests
EUR 831.6
Description: Enzyme Immunoassay for the Quantitative Determination of purified M13 Bacteriophage Particles

AAV5 Titration ELISA Kit (Fast)
AAV-A005H 96tests
EUR 706.2
Description: AAV5 Titration ELISA Kit(Fast) is based on ELISA sandwich method and designed to detect and quantitative determine of AAV5 capsid titration in AAV gene therapy product preparation processing. It contains AAV5 empty capsids and a pair of antibodies against AAV5. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of AAV5 capsid titration.

QuickTiter Retrovirus Quantitation Kit
VPK-120 20 assays
EUR 853.2
Description: Our QuickTiter Retrovirus Quantitation Kit provides a quick method for determining retroviral titer. This assay measures the viral nucleic acid content of your retrovirus, and may be performed either before or after purification of your virus.

Monkey Simian RetroVirus,SRV ELISA Kit
YX-SL0039Mk - Ask for price

Retrovirus Precipitation Solution
VC200 100 ml
EUR 194.04

Retrovirus Precipitation Solution
VC225 250 ml
EUR 432.6

Retrovirus Precipitation Solution
VC250 500 ml
EUR 814.8

Lentivirus and Retrovirus Purification Kit
K1458-100 100 Preps
EUR 1244.4

Lentivirus and Retrovirus Purification Kit
K1458-20 20 Preps
EUR 727.2

Retrovirus-related Gag Antibody
48261-100ul 100ul
EUR 399.6

Retrovirus-related Gag Antibody
48261-50ul 50ul
EUR 286.8

Rat Serum IgG detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80155-G 1 kit
EUR 781.2

Rat Serum IgM detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80155-M 1 kit
EUR 781.2

Mouse Serum IgM detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80150-M 1 kit
EUR 781.2

Mouse Serum IgG3 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80150-G3 1 kit
EUR 781.2

Retrovirus Concentration Solution (100 mL)
P905C - Ask for price

Endogenous retrovirus (replication competent) PCR kit
PCR-V725-48D 50T
EUR 510.96
Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent)

Endogenous retrovirus (replication competent) PCR kit
PCR-V725-96D 100T
EUR 625.8
Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent)

Endogenous retrovirus (replication competent) PCR kit
PCR-H725-48D 48T
EUR 543.6

Endogenous retrovirus (replication competent) PCR kit
PCR-H725-96D 96T
EUR 686.4

Mouse Primary Antibody (IgA) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80150-A 1 kit
EUR 781.2

Mouse Primary Antibody (IgE) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80150-E 1 kit
EUR 781.2

Rat Primary Antibody (IgA) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80155-A 1 kit
EUR 781.2

Rat Primary Antibody (IgE) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80155-E 1 kit
EUR 781.2

pCHAC-hKLF4 Retrovirus Vector Validated
ABP-SC-VCREKLF 10µg Ask for price