qPCR and One-Step RT MasterMixes

Viral RNA/DNA titer using qRT-PCR.

Virus Titration Kits

Our Titration Kits current a fast and straightforward approach for titrating your virus. The kits make use of each a quick RNA or DNA extraction step, and resolve viral RNA/DNA titer using qRT-PCR.

Advantages of abm’s titration kits:

  • No RNA purification — saves time and eliminates inaccuracy.
  • Ready-to-use reagent mix — reduces variability.
  • qRT-PCR in a single step — additional delicate and proper than completely different methods.
  • No NTC amplification — Distinctive primer design completely eliminates non-specific amplification in NTCs.

We moreover provide On the spot Lentivirus Titration Taking part in playing cards, which allow quick detection of lentiviral capsule proteins as a marker to substantiate the presence of lentivirus. Utilizing the lateral flow into immunoassay approach, viral titer yields are assessed instantly inside 10 minutes using our three step protocol.

he Retro-X qRT-PCR Titration Tools provides a fast and straightforward approach for measuring retrovirus titer. The kits use a quick RNA purification step sooner than quantifying the number of retroviral genome copies using qRT-PCR and TB Inexperienced utilized sciences.

qPCR and One-Step RT MasterMixes

qPCR and One-Step RT MasterMixes

Description

abm’s qPCR Retrovirus Titer Tools is a one-step assay by qPCR which does not require additional lysis or reverse transcription (RT) steps, and might be completed beneath one hour. Designed to ship extreme sensitivity and specificity, the tools ensures minimal non-specific background and better complete effectivity as compared with comparable kits within the market. ROX reference dye is obtainable separate from the MasterMix, making this tools universally appropriate with most qPCR gadgets.

PRODUCT COMPONENT QUANTITY
BlasTaq 2X qPCR Titer MasterMix 1.25 ml
Primer Mix 100 rxn (200 µl)
Commonplace Administration DNA 50 µl
ROX Reference Dye 15 µl
Nuclease-Free H2O 2 x 1.zero ml

 

SKU G949
Unit quantity 100 rxn
Storage State of affairs Retailer at -20°C

 

Quick willpower of retroviral titer

Whereas standard titration methods require as a lot as 10 days to complete, these titration kits require solely 4 hours and work with all MMLV-based retroviral vectors (not MSCV). Using qRT-PCR dramatically shortens the time interval between viral harvest and target-cell an an infection, allowing you to hold out every on the equivalent day.

Receive fixed retroviral transductions

A critical good thing about retroviral gene-delivery strategies is the facility to manage the extent of gene expression by adjusting the multiplicity of an an infection (MOI) to dictate the number of viral genomes that enter your objective cells. These titration kits allow you to make a quick correlation between the retroviral copy amount and retroviral infectivity in your cell type and in the long run allow you to administration the expression stage between experiments.

The best way it really works

The qRT-PCR course of entails subjecting serial dilutions of your sample and a administration RNA of acknowledged copy amount (provided) to one-step qRT-PCR. The retroviral copy amount contained in your sample can then be determined by evaluating its Ct value to the standard curve.

RNA purification and DNase I remedy steps are essential to the protocol so even viruses packaged using transient transfections, which always embrace residual plasmid DNA, might be exactly quantified using this tools.

 

Retrovirus Maxi Purification Kit
K1308-10
EUR 1295

Retrovirus Maxi Purification Kit
K1308-2
EUR 533

Retrovirus Maxi Purification Kit
K1308-4
EUR 860

QuickTiter Retrovirus Quantitation Kit
VPK-120 20 assays
EUR 711
Description: Our QuickTiter Retrovirus Quantitation Kit provides a quick method for determining retroviral titer. This assay measures the viral nucleic acid content of your retrovirus, and may be performed either before or after purification of your virus.

M13 Phage Titration ELISA Kit
55R-PRPHAGE 96 tests
EUR 693
Description: Enzyme Immunoassay for the Quantitative Determination of purified M13 Bacteriophage Particles

Retro-SV40LT Retrovirus
RVP010 1.0 ml
EUR 247

Retrovirus Booster 50µl
ABP-RVC-RBST05 50µl Ask for price

Retrovirus Booster 200µl
ABP-RVC-RBST20 200µl Ask for price

Retrovirus Booster 400µl
ABP-RVC-RBST40 400µl Ask for price

Retrovirus Stabilizer 50µl
ABP-RVC-RSTB05 50µl Ask for price

Retrovirus Stabilizer 200µl
ABP-RVC-RSTB20 200µl Ask for price

Retrovirus Stabilizer 400µl
ABP-RVC-RSTB40 400µl Ask for price

Lentivirus and Retrovirus Purification Kit
K1458-100 100 Preps
EUR 1037

Lentivirus and Retrovirus Purification Kit
K1458-20 20 Preps
EUR 606

qPCR Control Template Kit
G929 100 rxns
EUR 148

Lentivirus qPCR Quantification Kit
K1471-100 100 Rxns
EUR 298

QPCR Positive Control Kit
M1126-100
EUR 403

Eva QPCR SuperMix Kit
K5052200 200 reactions
EUR 211
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Eva QPCR SuperMix Kit
K5052400 400 reactions
EUR 320
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit
K5053200 200 reactions
EUR 188
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit
K5053400 400 reactions
EUR 299
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Entrans qPCR Probe Kit
RK21209 40 RXN Ask for price

Endogenous retrovirus (replication competent) PCR kit
PCR-H725-48D 48T
EUR 453

Endogenous retrovirus (replication competent) PCR kit
PCR-H725-96D 96T
EUR 572

Endogenous retrovirus (replication competent) PCR kit
PCR-V725-48D 50T
EUR 425.8
Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent)

Endogenous retrovirus (replication competent) PCR kit
PCR-V725-96D 100T
EUR 521.5
Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent)

ViraDuctin Retrovirus Transduction Kit (40 Transductions)
RV-200 40 transductions
EUR 363
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells.

ViraDuctin Retrovirus Transduction Kit (200 Transductions)
RV-201 200 transductions
EUR 780
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells.

iPS Cell Line (Retrovirus)
ASE-9101 1 vial of ^2.5X10^5 cells
EUR 430.7
Description: 6 month

Retrovirus-related Gag Antibody
48261-100ul 100ul
EUR 333

Retrovirus-related Gag Antibody
48261-50ul 50ul
EUR 239

Power Eva QPCR SuperMix Kit
K5057200 200 reactions
EUR 188
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Power Eva QPCR SuperMix Kit
K5057400 400 reactions
EUR 320
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Methylamp MS-qPCR Fast Kit
P-1028 200 Reactions
EUR 496.6
Description: fast delivery possible

Green qPCR SuperMix
20-abx098031
  • EUR 1038.00
  • EUR 439.00
  • EUR 634.00
  • 15 ml
  • 1 ml
  • 5 ml

Probe qPCR SuperMix
20-abx098040
  • EUR 1038.00
  • EUR 439.00
  • EUR 634.00
  • 15 ml
  • 1 ml
  • 5 ml

Universal qPCR Mixture
QPCR1000-UNIV 4X1.25ml
EUR 276.2

Frit Kit
FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles
RA900A-1 20 profiles
EUR 850

Column Packing Kit
PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.