Introduction: Unlike Escherichia coli , Campylobacter jejuni is unable to import a range of sugars, including arabinose, which makes common expression vectors, such as pBAD33, non-functional in these bacteria.
Aim: The aim of this study was to investigate whether the E. coli transporters AraE and modified LacY (LacYA177C) would enable C. jejuni to uptake arabinose.
Methodology and results: The respective genes of E. coli were constitutively expressed in C. jejuni strain 11168H after integration into the chromosome via homologous recombination. Vectors carrying these genes also contained a reporter gene, gfp, under the control of the arabinose-inducible promoter, pBAD. These constructs were verified in E. coli by demonstrating the induction of gfp in the presence of arabinose.
Integration of the genes into one of the rRNA gene clusters was verified by PCR and genome sequencing. The latter also confirmed that the inserted gene clusters contained no mutations. Expression of the gfp gene in the presence of arabinose inducer was monitored using fluorescence microscopy of colonies and fluorimetry using both whole cells and lysates.
Conclusion: The results demonstrated the inability of C. jejuni to use arabinose transporters, which are fully functional in E. coli , suggesting a remarkable difference in the physiology of these bacteria.
Genome-Wide Analysis of Peptidoglycan Recognition Protein Genes in Fig Wasps (Hymenoptera, Chalcidoidea)
- The innate immunity is the most important defense against pathogen of insects, and the peptidoglycan recognition proteins (PGRPs) play an important role in the processes of immune recognition and initiation of Toll, IMD and other signal pathways. In fig wasps, pollinators and non-pollinators present different evolutionary histories and lifestyles, even though both are closely associated with fig syconia, which may indicate their different patterns in the evolution of PGRPs.
- By manual annotation, we got all the PGRP genes of 12 fig wasp species, containing seven pollinators and five non-pollinators, and investigated their putative different evolutionary patterns. We found that the number of PGRP genes in pollinators was significantly lower than in non-pollinators, and the number of catalytic PGRP presented a declining trend in pollinators.
- More importantly, PGRP-SA is associated with initiating the Toll pathway, as well as gram-negative bacteria-binding proteins (GNBPs), which were completely lost in pollinators, which led us to speculate that the initiation of Toll pathway was simpler in pollinators than in non-pollinators.
- We concluded that fig pollinators owned a more streamlined innate immune recognition system than non-pollinators. Our results provide molecular evidence for the adaptive evolution of innate immunity in insects of host specificity.
Comparative genomics of Alexander Fleming’s original Penicillium isolate (IMI 15378) reveals sequence divergence of penicillin synthesis genes
Antibiotics were derived originally from wild organisms and therefore understanding how these compounds evolve among different lineages might help with the design of new antimicrobial drugs. We report the draft genome sequence of Alexander Fleming’s original fungal isolate behind the discovery of penicillin, now classified as Penicillium rubens Biourge (1923) (IMI 15378). We compare the structure of the genome and genes involved in penicillin synthesis with those in two ‘high producing’ industrial strains of P. rubens and the closely related species P. nalgiovense.
The main effector genes for producing penicillin G (pcbAB, pcbC and penDE) show amino acid divergence between the Fleming strain and both industrial strains, whereas a suite of regulatory genes are conserved. Homologs of penicillin N effector genes cefD1 and cefD2 were also found and the latter displayed amino acid divergence between the Fleming strain and industrial strains.
The draft assemblies contain several partial duplications of penicillin-pathway genes in all three P. rubens strains, to differing degrees, which we hypothesise might be involved in regulation of the pathway. The two industrial strains are identical in sequence across all effector and regulatory genes but differ in duplication of the pcbAB-pcbC-penDE complex and partial duplication of fragments of regulatory genes. We conclude that evolution in the wild encompassed both sequence changes of the effector genes and gene duplication, whereas human-mediated changes through mutagenesis and artificial selection led to duplication of the penicillin pathway genes.

stjosephs-hospital
Transposon Insertion in the purL Gene Induces Biofilm Depletion in Escherichia coli ATCC 25922
Current Escherichia coli antibiofilm treatments comprise a combination of antibiotics commonly used against planktonic cells, leading to treatment failure. A better understanding of the genes involved in biofilm formation could facilitate the development of efficient and specific new antibiofilm treatments.
A total of 2578 E. coli mutants were generated by transposon insertion, of which 536 were analysed in this study. After sequencing, Tn263 mutant, classified as low biofilm-former (LF) compared to the wild-type (wt) strain (ATCC 25922), showed an interruption in the purL gene, involved in the de novo purine biosynthesis pathway.
To elucidate the role of purL in biofilm formation, a knockout was generated showing reduced production of curli fibres, leading to an impaired biofilm formation. These conditions were restored by complementation of the strain or addition of exogenous inosine. Proteomic and transcriptional analyses were performed to characterise the differences caused by purL alterations.
Thirteen proteins were altered compared to wt. The corresponding genes were analysed by qRT-PCR not only in the Tn263 and wt, but also in clinical strains with different biofilm activity. Overall, this study suggests that purL is essential for biofilm formation in E. coli and can be considered as a potential antibiofilm target.
Characterizing patient-oncologist communication in genomic tumor testing: The 21-gene recurrence score as an exemplar
Objective: Women with early-stage, ER + breast cancer are recommend to receive genomic profiling tests, such as the 21-gene Recurrence Score (RS) test, to guide treatment decisions. We examined test- and treatment-related information discussed and the associations between RS categories and aspects of communication during patient-oncologist clinical encounters.
Methods: As part of a larger trial, clinical encounters (N = 46) were audiorecorded and coded for 1) RS- and treatment-related information, 2) shared decision making, 3) patient active participation, and 4) oncologist patient-centered communication. We examined differences by RS category using mixed models, adjusting for nesting within oncologist.
Results: Patients with a high RS were more likely to receive a chemotherapy recommendation (p < .01), hear about the risks/side effects of chemotherapy (p < .01), and offer their preferences (p = .02) than those with intermediate or low RS. Elements of shared decision making increased with RS. Oncologist patient-centered communication (M = 4.09/5, SD = .25) and patient active participation (M = 3.5/4, SD = 1.0) were high across RS.
Conclusion: Findings suggest that disease severity, rather than clinical uncertainty, impact treatment recommendations and shared decision making.
Practice implications: Oncologists adjust test- and treatment-related information and shared decision making by disease severity. This information provides a framework to inform decision making in complex cancer and genomics settings.
Human Actinin Alpha 3 (ACTN3) ELISA Kit |
DLR-ACTN3-Hu-48T |
DL Develop |
48T |
EUR 498 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Actinin Alpha 3 (ACTN3) in samples from tissue homogenates, cell lysates or other biological fluids. |
Human Actinin Alpha 3 (ACTN3) ELISA Kit |
DLR-ACTN3-Hu-96T |
DL Develop |
96T |
EUR 647 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Actinin Alpha 3 (ACTN3) in samples from tissue homogenates, cell lysates or other biological fluids. |
Human Actinin Alpha 3 (ACTN3) ELISA Kit |
RD-ACTN3-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 500 |
Human Actinin Alpha 3 (ACTN3) ELISA Kit |
RD-ACTN3-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 692 |
Human Actinin Alpha 3 (ACTN3) ELISA Kit |
RDR-ACTN3-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 522 |
Human Actinin Alpha 3 (ACTN3) ELISA Kit |
RDR-ACTN3-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 724 |
Rabbit Polyclonal antibody Anti-CRBN |
Anti-CRBN |
ImmunoStep |
50 µg |
EUR 349 |
Anti-ACTN3 Antibody (monoclonal, 9B5) |
M02693 |
BosterBio |
100ug/vial |
EUR 334 |
ACTN3 Antibody |
34157-100ul |
SAB |
100ul |
EUR 252 |
ACTN3 Antibody |
34157-50ul |
SAB |
50ul |
EUR 187 |
ACTN3 Antibody |
1-CSB-PA124802 |
Cusabio |
|
|
|
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000 |
ACTN3 Antibody |
1-CSB-PA288460 |
Cusabio |
|
|
|
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000 |
ACTN3 Antibody |
1-CSB-PA000816 |
Cusabio |
|
|
|
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/20000 |
ACTN3 Antibody |
1-CSB-PA001243LA01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200 |
ACTN3 Antibody |
DF8016 |
Affbiotech |
200ul |
EUR 304 |
Description: ACTN3 Antibody detects endogenous levels of total ACTN3. |
ACTN3 antibody |
70R-32269 |
Fitzgerald |
100 ug |
EUR 327 |
Description: Rabbit polyclonal ACTN3 antibody |
ACTN3 antibody |
70R-49332 |
Fitzgerald |
100 ul |
EUR 244 |
Description: Purified Polyclonal ACTN3 antibody |
Polyclonal ACTN3 Antibody |
APR14796G |
Leading Biology |
0.1ml |
EUR 484 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ACTN3 . This antibody is tested and proven to work in the following applications: |
ACTN2 / ACTN3 Antibody |
20-abx329446 |
Abbexa |
|
|
|
ACTN2 / ACTN3 Antibody |
abx332374-100ul |
Abbexa |
100 ul |
EUR 425 |
|
ACTN2/ACTN3 Antibody |
CSB-PA960396- |
Cusabio |
|
EUR 335 |
|
Description: A polyclonal antibody against ACTN2/ACTN3. Recognizes ACTN2/ACTN3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 |
ACTN2/ACTN3 Antibody |
CSB-PA960396-100ul |
Cusabio |
100ul |
EUR 316 |
|
Description: A polyclonal antibody against ACTN2/ACTN3. Recognizes ACTN2/ACTN3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 |
ACTN2/ACTN3 Antibody |
1-CSB-PA000815 |
Cusabio |
|
|
|
Description: A polyclonal antibody against ACTN2/ACTN3. Recognizes ACTN2/ACTN3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/20000 |
ACTN3 Conjugated Antibody |
C34157 |
SAB |
100ul |
EUR 397 |
ACTN3 siRNA |
20-abx906644 |
Abbexa |
|
|
|
ACTN3 siRNA |
20-abx906645 |
Abbexa |
|
|
|
Polyclonal ACTN3 Antibody (Center) |
APR14798G |
Leading Biology |
0.1ml |
EUR 484 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ACTN3 (Center). This antibody is tested and proven to work in the following applications: |
ACTN3 Antibody, HRP conjugated |
1-CSB-PA001243LB01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
ACTN3 Antibody, FITC conjugated |
1-CSB-PA001243LC01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
ACTN3 Antibody, Biotin conjugated |
1-CSB-PA001243LD01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
Anti-Human ACTN3 DyLight® 488 conjugated Antibody |
A02693-Dyl488 |
BosterBio |
100ug/vial |
EUR 344 |
Anti-Human ACTN3 DyLight® 550 conjugated Antibody |
A02693-Dyl550 |
BosterBio |
100ug/vial |
EUR 344 |
Polyclonal Goat anti-GST α-form |
GST-ANTI-1 |
Detroit R&D |
50 uL |
EUR 280 |
Polyclonal Goat anti-GST μ-form |
GST-ANTI-2 |
Detroit R&D |
50 uL |
EUR 280 |
Polyclonal Goat anti-GST p-form |
GST-ANTI-3 |
Detroit R&D |
50 uL |
EUR 280 |
ACTN3 Blocking Peptide |
20-abx062207 |
Abbexa |
|
|
|
ACTN3 Rabbit pAb |
A12797-100ul |
Abclonal |
100 ul |
EUR 308 |
ACTN3 Rabbit pAb |
A12797-200ul |
Abclonal |
200 ul |
EUR 459 |
ACTN3 Rabbit pAb |
A12797-20ul |
Abclonal |
20 ul |
EUR 183 |
ACTN3 Rabbit pAb |
A12797-50ul |
Abclonal |
50 ul |
EUR 223 |
ACTN3 Rabbit pAb |
A12864-100ul |
Abclonal |
100 ul |
EUR 308 |
ACTN3 Rabbit pAb |
A12864-200ul |
Abclonal |
200 ul |
EUR 459 |
ACTN3 Rabbit pAb |
A12864-20ul |
Abclonal |
20 ul |
EUR 183 |
ACTN3 Rabbit pAb |
A12864-50ul |
Abclonal |
50 ul |
EUR 223 |
ACTN3 Blocking Peptide |
DF8016-BP |
Affbiotech |
1mg |
EUR 195 |
Actinin Alpha 3 (ACTN3) Antibody |
20-abx130639 |
Abbexa |
-
EUR 411.00
-
EUR 133.00
-
EUR 1149.00
-
EUR 565.00
-
EUR 314.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Actinin Alpha 3 (ACTN3) Antibody |
abx038105-100ug |
Abbexa |
100 ug |
EUR 391 |
|
Actinin Alpha 3 (ACTN3) Antibody |
20-abx009624 |
Abbexa |
-
EUR 300.00
-
EUR 439.00
-
EUR 189.00
|
|
|
Actinin Alpha 3 (ACTN3) Antibody |
20-abx013892 |
Abbexa |
-
EUR 314.00
-
EUR 98.00
-
EUR 398.00
-
EUR 495.00
|
-
100 ug
-
10 ug
-
200 ug
-
300 µg
|
|
Actinin Alpha 3 (ACTN3) Antibody |
abx026732-400ul |
Abbexa |
400 ul |
EUR 523 |
|
Actinin Alpha 3 (ACTN3) Antibody |
abx026732-80l |
Abbexa |
80 µl |
EUR 286 |
|
Actinin Alpha 3 (ACTN3) Antibody |
abx230122-100ug |
Abbexa |
100 ug |
EUR 509 |
|
Alpha Actinin 3 (ACTN3) Antibody |
20-abx213418 |
Abbexa |
|
|
|
Alpha Actinin 3 (ACTN3) Antibody |
20-abx213419 |
Abbexa |
|
|
|
Polyclonal ACTN3 Antibody (aa1-50) |
APR14797G |
Leading Biology |
0.05ml |
EUR 484 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ACTN3 (aa1-50). This antibody is tested and proven to work in the following applications: |
ACTN1 / ACTN2 / ACTN3 / ACTN4 Antibody |
abx332562-100ul |
Abbexa |
100 ul |
EUR 425 |
|
Alpha Actinin 3 (ACTN3) Antibody |
20-abx328117 |
Abbexa |
|
|
|
ACTN1 / ACTN2 / ACTN3 / ACTN4 Antibody |
20-abx328684 |
Abbexa |
|
|
|
ACTN1/ACTN2/ACTN3/ACTN4 Antibody |
CSB-PA096347- |
Cusabio |
|
EUR 335 |
|
Description: A polyclonal antibody against ACTN1/ACTN2/ACTN3/ACTN4. Recognizes ACTN1/ACTN2/ACTN3/ACTN4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
ACTN1/ACTN2/ACTN3/ACTN4 Antibody |
CSB-PA096347-100ul |
Cusabio |
100ul |
EUR 316 |
|
Description: A polyclonal antibody against ACTN1/ACTN2/ACTN3/ACTN4. Recognizes ACTN1/ACTN2/ACTN3/ACTN4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
ACTN1/ACTN2/ACTN3/ACTN4 Antibody |
1-CSB-PA000814 |
Cusabio |
|
|
|
Description: A polyclonal antibody against ACTN1/ACTN2/ACTN3/ACTN4. Recognizes ACTN1/ACTN2/ACTN3/ACTN4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000 |
Mouse ACTN3 ELISA Kit |
EMA0390 |
Abclonal |
96Tests |
EUR 521 |
Porcine ACTN3 ELISA Kit |
EPA0390 |
Abclonal |
96Tests |
EUR 521 |
Rat ACTN3 ELISA Kit |
ERA0390 |
Abclonal |
96Tests |
EUR 521 |
Rabbit ACTN3 ELISA Kit |
ERTA0390 |
Abclonal |
96Tests |
EUR 521 |
Anserini ACTN3 ELISA Kit |
EAA0390 |
Abclonal |
96Tests |
EUR 521 |
Goat ACTN3 ELISA Kit |
EGTA0390 |
Abclonal |
96Tests |
EUR 521 |
Canine ACTN3 ELISA Kit |
ECA0390 |
Abclonal |
96Tests |
EUR 521 |
Human ACTN3 ELISA Kit |
EHA0390 |
Abclonal |
96Tests |
EUR 521 |
Bovine ACTN3 ELISA Kit |
EBA0390 |
Abclonal |
96Tests |
EUR 521 |
Human ACTN3 shRNA Plasmid |
20-abx950055 |
Abbexa |
|
|
|
Mouse ACTN3 shRNA Plasmid |
20-abx969019 |
Abbexa |
|
|
|
Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human) |
4-PAB104Hu01 |
Cloud-Clone |
-
EUR 239.00
-
EUR 2391.00
-
EUR 598.00
-
EUR 299.00
-
EUR 211.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3) |
Guinea Pig ACTN3 ELISA Kit |
EGA0390 |
Abclonal |
96Tests |
EUR 521 |
ACTN3 ELISA Kit (Human) (OKEH04449) |
OKEH04449 |
Aviva Systems Biology |
96 Wells |
EUR 662 |
Description: Description of target: This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 4.1 pg/mL |
Recombinant Actinin Alpha 3 (ACTN3) |
4-RPB104Hu01 |
Cloud-Clone |
-
EUR 458.40
-
EUR 226.00
-
EUR 1444.00
-
EUR 548.00
-
EUR 996.00
-
EUR 370.00
-
EUR 3460.00
|
-
100 ug
-
10ug
-
1 mg
-
200 ug
-
500 ug
-
50ug
-
5 mg
|
|
Description: Recombinant Human Actinin Alpha 3 expressed in: E.coli |
ACTN3 ELISA Kit (Mouse) (OKEH05687) |
OKEH05687 |
Aviva Systems Biology |
96 Wells |
EUR 662 |
Description: Description of target: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 41 pg/mL |
ACTN3 ORF Vector (Human) (pORF) |
ORF012141 |
ABM |
1.0 ug DNA |
EUR 354 |
Actn3 ORF Vector (Mouse) (pORF) |
ORF038023 |
ABM |
1.0 ug DNA |
EUR 506 |
Actn3 ORF Vector (Rat) (pORF) |
ORF063016 |
ABM |
1.0 ug DNA |
EUR 506 |
Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human), APC |
4-PAB104Hu01-APC |
Cloud-Clone |
-
EUR 333.00
-
EUR 3113.00
-
EUR 872.00
-
EUR 423.00
-
EUR 215.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3). This antibody is labeled with APC. |
Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human), Biotinylated |
4-PAB104Hu01-Biotin |
Cloud-Clone |
-
EUR 303.00
-
EUR 2341.00
-
EUR 697.00
-
EUR 369.00
-
EUR 216.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3). This antibody is labeled with Biotin. |
Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human), Cy3 |
4-PAB104Hu01-Cy3 |
Cloud-Clone |
-
EUR 403.00
-
EUR 4109.00
-
EUR 1121.00
-
EUR 523.00
-
EUR 245.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3). This antibody is labeled with Cy3. |
Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human), FITC |
4-PAB104Hu01-FITC |
Cloud-Clone |
-
EUR 287.00
-
EUR 2510.00
-
EUR 717.00
-
EUR 359.00
-
EUR 192.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3). This antibody is labeled with FITC. |
Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human), HRP |
4-PAB104Hu01-HRP |
Cloud-Clone |
-
EUR 305.00
-
EUR 2714.00
-
EUR 772.00
-
EUR 383.00
-
EUR 203.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3). This antibody is labeled with HRP. |