Acute myocardial infarction (AMI) seriously threatens human life. In this study we aimed to systemically analyze the function of key gene modules in human platelets in AMI. We used weighted gene co-expression network analysis (WGCNA) to construct a co-expression module, and analyzed the relationship between potential modules and clinical characteristics based on platelet RNA-seq RPKM count reads of 16 ST-segment elevation myocardial infarction (STEMI) patients and 16 non-STEMI (NSTEMI) patients provided by the GEO database.

Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed with the DAVID tool. Hub genes were calculated by the Cytohubba package. A total of 3653 genes was selected to construct the co-expression modules.

A significant correlation between BMI and the module with color of sky-blue in STEMI. In NSTEMI, there was a significant correlation between the sky blue module and CAD, the Salmon module and HT, and the Cyan module and HT. In STEMI, the Hub genes were mainly enriched in functions related to cell membrane signal transduction including Aqp1, Armcx1, Gsta4, Hist3h2a and Il17re.

In NSTEMI, the Hub genes are related mainly to energy metabolism in the sky-blue module including Olr1, Nap1l3, Gfer, Dohh, Crispld1 and Ccdc8b; they are mainly related to extracellular space and calcium binding in the Cyan module, including Clec12b, Chd4, Asgr1, Armcx4, Chid1 and Alkbh7.

The hub genes in the Salmon module include Ell3, Aldh1b1, Cavin4, Cabp4, Eif1ay and Dus3l. Our results provide a framework for co-expression gene modules in STEMI and NSTEMI patients, and identify key targets as biomarkers for patients with different subtypes of AMI.

Reproducibility and sensitivity of 36 methods to quantify the SARS-CoV-2 genetic signal in raw wastewater: findings from an interlaboratory methods evaluation in the U.S

In response to COVID-19, the international water community rapidly developed methods to quantify the SARS-CoV-2 genetic signal in untreated wastewater. Wastewater surveillance using such methods has the potential to complement clinical testing in assessing community health. This interlaboratory assessment evaluated the reproducibility and sensitivity of 36 standard operating procedures (SOPs), divided into eight method groups based on sample concentration approach and whether solids were removed.

Two raw wastewater samples were collected in August 2020, amended with a matrix spike (betacoronavirus OC43), and distributed to 32 laboratories across the U.S. Replicate samples analyzed in accordance with the project’s quality assurance plan showed high reproducibility across the 36 SOPs: 80% of the recovery-corrected results fell within a band of ±1.15 log₁₀ genome copies per L with higher reproducibility observed within a single SOP (standard deviation of 0.13 log₁₀).

The inclusion of a solids removal step and the selection of a concentration method did not show a clear, systematic impact on the recovery-corrected results. Other methodological variations (e.g., pasteurization, primer set selection, and use of RT-qPCR or RT-dPCR platforms) generally resulted in small differences compared to other sources of variability.

These findings suggest that a variety of methods are capable of producing reproducible results, though the same SOP or laboratory should be selected to track SARS-CoV-2 trends at a given facility. The methods showed a 7 log₁₀ range of recovery efficiency and limit of detection highlighting the importance of recovery correction and the need to consider method sensitivity when selecting methods for wastewater surveillance.

ExTraMapper: Exon- and Transcript-level mappings for orthologous gene pairs

Motivation: Access to large-scale genomics and transcriptomics data from various tissues and cell lines allowed the discovery of wide-spread alternative splicing events and alternative promoter usage in mammalians. Between human and mouse, gene-level orthology is currently present for nearly 16k protein-coding genes spanning a diverse repertoire of over 200k total transcript isoforms.

Results: Here, we describe a novel method, ExTraMapper, which leverages sequence conservation between exons of a pair of organisms and identifies a fine-scale orthology mapping at the exon and then transcript level. ExTraMapper identifies more than 350k exon mappings, as well as 30k transcript mappings between human and mouse using only sequence and gene annotation information.

We demonstrate that ExTraMapper identifies a larger number of exon and transcript mappings compared to previous methods. Further, it identifies exon fusions, splits, and losses due to splice site mutations, and finds mappings between microexons that are previously missed.

By reanalysis of RNA-seq data from 13 matched human and mouse tissues, we show that ExTraMapper improves the correlation of transcript-specific expression levels suggesting a more accurate mapping of human and mouse transcripts. We also applied the method to detect conserved exon and transcript pairs between human and rhesus macaque genomes to highlight the point that ExTraMapper is applicable to any pair of organisms that have orthologous gene pairs.

Availability: The source code and the results are available

Unconventional viral gene expression mechanisms as therapeutic targets

Unlike the human genome that comprises mostly noncoding and regulatory sequences, viruses have evolved under the constraints of maintaining a small genome size while expanding the efficiency of their coding and regulatory sequences.

As a result, viruses use strategies of transcription and translation in which one or more of the steps in the conventional gene-protein production line are altered. These alternative strategies of viral gene expression (also known as gene recoding) can be uniquely brought about by dedicated viral enzymes or by co-opting host factors (known as host dependencies).

Targeting these unique enzymatic activities and host factors exposes vulnerabilities of a virus and provides a paradigm for the design of novel antiviral therapies. In this Review, we describe the types and mechanisms of unconventional gene and protein expression in viruses, and provide a perspective on how future basic mechanistic work could inform translational efforts that are aimed at viral eradication.

 

GCNF Antibody
DF13790	Affbiotech	100ul	EUR 420

GCNF Antibody
DF13790-100ul	Affinity Biosciences	100ul	EUR 280

GCNF Antibody
DF13790-200ul	Affinity Biosciences	200ul	EUR 350

GCNF Antibody
MBS9435332-005mL	MyBiosource	0.05mL	EUR 325

GCNF Antibody
MBS9435332-01mL	MyBiosource	0.1mL	EUR 445

GCNF Antibody
MBS9435332-5x01mL	MyBiosource	5x0.1mL	EUR 1860

GCNF Antibody
MBS9631462-1mg	MyBiosource	1mg	EUR 375

GCNF Antibody
MBS9631462-5x1mg	MyBiosource	5x1mg	EUR 1545

GCNF Antibody
MBS9629079-01mL	MyBiosource	0.1mL	EUR 260

GCNF Antibody
MBS9629079-02mL	MyBiosource	0.2mL	EUR 305

GCNF Antibody
MBS9629079-5x02mL	MyBiosource	5x0.2mL	EUR 1220

anti- GCNF antibody
FNab03390	FN Test	100µg	EUR 606.3
Description: Antibody raised against GCNF

GCNF Conjugated Antibody
C48479	SAB	100ul	EUR 476.4

GCNF Conjugated Antibody
MBS9456445-01mLAF350	MyBiosource	0.1mL(AF350)	EUR 480

GCNF Conjugated Antibody
MBS9456445-01mLAF405	MyBiosource	0.1mL(AF405)	EUR 480

GCNF Conjugated Antibody
MBS9456445-01mLAF488	MyBiosource	0.1mL(AF488)	EUR 480

GCNF Conjugated Antibody
MBS9456445-01mLAF555	MyBiosource	0.1mL(AF555)	EUR 480

GCNF Conjugated Antibody
MBS9456445-01mLBiotin	MyBiosource	0.1mL(Biotin)	EUR 480

Germ Cell Nuclear Factor (GCNF) Antibody
20-abx131389	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 10 ug 1 mg 200 ug 50 ug

Germ Cell Nuclear Factor (GCNF) Antibody
abx131389-02mg	Abbexa	0.2 mg	EUR 337.5

Germ Cell Nuclear Factor (GCNF) Antibody
abx131389-05mg	Abbexa	0.5 mg	EUR 750

Germ Cell Nuclear Factor (GCNF) Antibody
abx131389-100g	Abbexa	100 µg	EUR 275

Polyclonal NR6A1 / GCNF Antibody (Ligand-binding Domain)
AMM06820G	Leading Biology	0.05mg	EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human NR6A1 / GCNF (Ligand-binding Domain). This antibody is tested and proven to work in the following applications:

GCNF (anti human nuclear recepter antibody, clone# H7921)
PP-H7921-00	Sceti	0.1mg/100uL	EUR 747.6
Description: The GCNF (anti human nuclear recepter antibody, clone# H7921) is available in Europe and for worldwide shipping via Gentaur.

GCNF
E8EM1710-12	EnoGene	100ul	EUR 275
Description: Available in various conjugation types.

GCNF
MBS8565176-01mL	MyBiosource	0.1mL	EUR 345

GCNF
MBS8565176-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

GCNF
MBS8565176-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

GCNF
MBS8565176-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

GCNF
MBS8565176-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody
CAU24184-100ul	Biomatik Corporation	100ul	EUR 235.2

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody
CAU24184-200ul	Biomatik Corporation	200ul	EUR 294

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody
MBS2032347-01mL	MyBiosource	0.1mL	EUR 175

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody
MBS2032347-02mL	MyBiosource	0.2mL	EUR 220

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody
MBS2032347-05mL	MyBiosource	0.5mL	EUR 355

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody
MBS2032347-1mL	MyBiosource	1mL	EUR 435

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody
MBS2032347-5mL	MyBiosource	5mL	EUR 1185

Human Germ Cell Nuclear Factor (GCNF) Antibody Pair Kit (with Standard)
MBS2101752-10x96Tests	MyBiosource	10x96Tests	EUR 1480

Human Germ Cell Nuclear Factor (GCNF) Antibody Pair Kit (with Standard)
MBS2101752-10x96TestsMBS2090685AbPairsSupportPack110x96Tests	MyBiosource	10x96Tests+MBS2090685(AbPairsSupportPack1,10x96Tests)	EUR 1600

Human Germ Cell Nuclear Factor (GCNF) Antibody Pair Kit (with Standard)
MBS2101752-5x96Tests	MyBiosource	5x96Tests	EUR 935

Human Germ Cell Nuclear Factor (GCNF) Antibody Pair Kit (with Standard)
MBS2101752-5x96TestsMBS2090685AbPairsSupportPack15x96Tests	MyBiosource	5x96Tests+MBS2090685(AbPairsSupportPack1,5x96Tests)	EUR 1010

Polyclonal Antibody to Germ Cell Nuclear Factor (GCNF)
PAC509Hu01	Cloud-Clone	100ul	EUR 245

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human)
4-PAC509Hu01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF)

NR6A1 / GCNF Rabbit Polyclonal (Ligand-binding Domain) Antibody
TA317092	Origene Technologies GmbH	50 µg	Ask for price

NR6A1 / GCNF Rabbit Polyclonal (Ligand-binding Domain) Antibody
TA317093	Origene Technologies GmbH	50 µg	Ask for price

GCNF Mouse mAb
E2220732	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

GCNF Mouse mAb
MBS8538939-01mL	MyBiosource	0.1mL	EUR 305

GCNF Mouse mAb
MBS8538939-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

GCNF Mouse mAb
MBS8538939-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

GCNF Mouse mAb
MBS8538939-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

GCNF Mouse mAb
MBS8538939-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human), PE
4-PAC509Hu01-PE	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with PE.

GCNF (NR6A1) (N-term) rabbit polyclonal antibody, Aff - Purified
AP52935PU-N	Origene Technologies GmbH	400 µl	Ask for price

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human), APC
4-PAC509Hu01-APC	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with APC.

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human), Cy3
4-PAC509Hu01-Cy3	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with Cy3.

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human), HRP
4-PAC509Hu01-HRP	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with HRP.

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human), FITC
4-PAC509Hu01-FITC	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with FITC.

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human), Biotinylated
4-PAC509Hu01-Biotin	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with Biotin.

PE-Linked Polyclonal Antibody to Germ Cell Nuclear Factor (GCNF)
MBS2066290-01mL	MyBiosource	0.1mL	EUR 265

PE-Linked Polyclonal Antibody to Germ Cell Nuclear Factor (GCNF)
MBS2066290-02mL	MyBiosource	0.2mL	EUR 355

PE-Linked Polyclonal Antibody to Germ Cell Nuclear Factor (GCNF)
MBS2066290-05mL	MyBiosource	0.5mL	EUR 660

PE-Linked Polyclonal Antibody to Germ Cell Nuclear Factor (GCNF)
MBS2066290-1mL	MyBiosource	1mL	EUR 810

PE-Linked Polyclonal Antibody to Germ Cell Nuclear Factor (GCNF)
MBS2066290-5mL	MyBiosource	5mL	EUR 2330

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human), APC-Cy7
4-PAC509Hu01-APC-Cy7	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with APC-Cy7.

APC-Linked Polyclonal Antibody to Germ Cell Nuclear Factor (GCNF)
MBS2066291-01mL	MyBiosource	0.1mL	EUR 265

APC-Linked Polyclonal Antibody to Germ Cell Nuclear Factor (GCNF)
MBS2066291-02mL	MyBiosource	0.2mL	EUR 355

APC-Linked Polyclonal Antibody to Germ Cell Nuclear Factor (GCNF)
MBS2066291-05mL	MyBiosource	0.5mL	EUR 660

APC-Linked Polyclonal Antibody to Germ Cell Nuclear Factor (GCNF)
MBS2066291-1mL	MyBiosource	1mL	EUR 810