The Total Exosome RNA and Protein Isolation Kit enables isolation of total RNA and proteins from exosome samples extracted from biological fluids (e.g., by using Total Exosome Isolation (from cell culture media) or Total Exosome Isolation (from serum)).
• Extracts highly pure total RNA (including small RNA fraction) from exosomes
• Recovers protein and RNA from the same sample
• Compatible with all protocols for exosome isolation
The Total Exosome RNA and Protein Isolation Kit is designed for the isolation of both RNA and protein from pre-isolated or enriched exosomes (not supplied). It is suitable for studies of RNA expression (specifically miRNA), processing, or function. The kit allows recovery of both RNA and proteins from the same purified exosome preparation. One portion of the sample is subjected to organic extraction, followed by immobilization of RNA on glass-fiber filters to purify total RNA or enrich for small RNA species. The other portion is then used directly for common protein applications such as Western blotting.
High yields of ultra-pure RNA can be prepared in about 30–60 minutes. The RNA can then be used in a variety of downstream applications, including qRT-PCR, high-throughput sequencing (e.g., using the Ion Torent™ PGM™, Ion Torrent™ Proton™, or SOLiD sequencing instruments), microarray analysis, solution hybridization assays, and blot hybridization.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
exoEasy Maxi Kit
For purification of exosomes and other extracellular vesicles (EVs) from plasma, serum and cell culture supernatant
Features
- Isolate intact exosomes and other extracellular vesicles
- Suitable for functional testing and physical and biochemical analysis
- Purify exosomes and other extracellular vesicles in 25 minutes
- Process multiple samples in parallel using a simple spin-column procedure
- Use with up to 4 ml of plasma or serum or 32 ml of cell culture supernatant
- Suitable for MISEV-compliant protein characterization
Product Details
The exoEasy Maxi Kit uses membrane affinity spin columns to efficiently isolate exosomes and other extracellular vesicles from serum, plasma, cell culture supernatant and other biological fluids. The maxi column format allows the use of large sample volumes, including up to 4 ml plasma or serum or up to 32 ml cell culture supernatant. The procedure is very fast, consistent and highly suited for isolation of EVs for functional analysis of exosomes and other extracellular vesicles.
buffer a exosome protein extraction kit
Performance
 Exosome RNA)
 Exosome RNA)