The impact of cross-kingdom molecular forensics on genetic privacy

stjosephs-hospital

Recent advances in metagenomic technology and computational prediction may inadvertently weaken an individual’s reasonable expectation of privacy. Through cross-kingdom genetic and metagenomic forensics, we can already predict at least a dozen human phenotypes with varying degrees of accuracy.

There is also growing potential to detect a “molecular echo” of an individual’s microbiome from cells deposited on public surfaces. At present, host genetic data from somatic or germ cells provide more reliable information than microbiome samples.

However, the emerging ability to infer personal details from different microscopic biological materials left behind on surfaces requires in-depth ethical and legal scrutiny. There is potential to identify and track individuals, along with new, surreptitious means of genetic discrimination.

This commentary underscores the need to update legal and policy frameworks for genetic privacy with additional considerations for the information that could be acquired from microbiome-derived data. The article also aims to stimulate ubiquitous discourse to ensure the protection of genetic rights and liberties in the post-genomic era. Video abstract.

MiR-340 promotes the proliferation of vascular smooth muscle cells by targeting von Hippel-Lindau tumor suppressor (VHL) gene

MiRNAs play key roles in the proliferation of vascular smooth muscle cells (VSMCs). However, the roles and underlying mechanism of miRNAs in VSMCs are not fully understood. The aim of this study was to evaluate the role of miR-340 in the proliferation of VSMCs.

The expression levels of miR-340 and von Hippel-Lindau tumor-suppressor (VHL) in VSMCs induced by platelet-derived growth factor (PDGF) -BB or fetal bovine serum (FBS) were measured by q-PCR. The effects of miR-340 and VHL on cell proliferation and invasion were evaluated by CCK-8 assay. Target gene prediction and screening as well as luciferase reporter assay were performed to verify the downstream target genes of miR-340. Western blotting was used to detect the protein expression levels of vascular endothelial growth factor (VEGF) and VHL.

Our results showed that the miR-340 was up-regulated in PDGF-BB_ENREF_1or FBS induced VSMCs. In addition, overexpression of miR-340 promoted VSMCs proliferation and invasion. Moreover, VHL was found to be a potential target for miR-340, and up-regulation of VHL inhibited VSMCs proliferation.

MiR-340 plays a critical role in VSMC proliferation and neointimal hyperplasia in rats carotid balloon injury model. Reduced expression levels of miR-340 promoted VHL-inhibited VSMCs proliferation. In conclusion, miR-340 may play a role in the regulation of proliferation of VSMCs by inhibition of VHL.

 

ECM2 and GLT8D2 in human pulmonary artery hypertension: fruits from weighted gene co-expression network analysis

Background: Pulmonary artery hypertension (PAH) is an incurable disease with a high mortality rate. Current medications ameliorate symptoms but cannot target adverse vascular remodeling. New therapeutic strategies for PAH need to be established.

 

Methods: Using the weighted gene coexpression network analysis (WGCNA) algorithm, we constructed a coexpression network of dataset GSE117261 from the Gene Expression Omnibus (GEO) database. Key modules were identified by the relationship between module eigengenes and clinical traits.

 

Hub genes were screened out based on gene significance (GS), module membership (MM), and mean pulmonary artery pressure (mPAP). External validations were conducted in GSE48149 and GSE113439. Functional enrichment and immune cell infiltration were analyzed using Metascape and CIBERSORTx.

Results: The WGCNA analysis revealed 13 coexpression modules. The pink module had the highest correlation with PAH in terms of module eigengene (r=0.79; P=2e-18) and module significance (MS =0.43).

 

Functional enrichment indicated genes in the pink module contributed to the immune system process and extracellular matrix (ECM). In the pink module, ECM2 (GS =0.65, MM =0.86, ρ=0.407, P=0.0019) and GLT8D2 (GS =0.63, MM =0.85, ρ=0.443, P=0.006) were identified as hub genes. For immune cells infiltration in PAH lung tissue, hub genes were positively correlated with M2 macrophages and resting mast cells, and were negatively correlated with monocytes, neutrophils, and CD4-naïve T cells.

 

Conclusions: Our research identified 2 hub genes ECM2 and GLT8D2 related to PAH. The functions of these hub genes were involved in the immune process and ECM, indicating that they might serve as candidate therapeutic targets for PAH.

stjosephs-hospital

stjosephs-hospital

Genomes of 12 fig wasps provide insights into the adaptation of pollinators to fig syconia

Figs and fig pollinators are one of the few classic textbook examples of obligate pollination mutualism. The specific dependence of fig pollinators on the relatively safe living environment with sufficient food sources in the enclosed fig syconia implies that they are vulnerable to habitat changes.

 

  • However, there is still no extensive genomic evidence to reveal the evolutionary footprint of this long-term mutually beneficial symbiosis in fig pollinators. In fig syconia, there are also non-pollinator species.
  • The non-pollinator species differ in their evolutionary and life histories from pollinators. We conducted comparative analyses on 11 newly sequenced fig wasp genomes and one previously published genome.
  • The pollinators colonized the figs approximately 66.9 million years ago, consistent with the origin of host figs. Compared with non-pollinators, many more genes in pollinators were subject to relaxed selection.
  • Seven genes were absent in pollinators in response to environmental stress and immune activation. Pollinators had more streamlined gene repertoires in the innate immune system, chemosensory toolbox, and detoxification system.
  • Our results provide genomic evidence for the differentiation between pollinators and nonpollinators. The data suggest that owing to the long-term adaptation to the fig, some genes related to functions no longer required are absent in pollinators.

Byrsonima Rich. is one of the largest genera of the Malpighiaceae family with 97 species occurrence in Brazil and multiple potentialities, including pharmaceutical and food industries. In this study, 17 microsatellite markers characterized in Byrsonima cydoniifolia were tested for seven related taxa, all species are native to Brazil and four are endemic. Genomic DNA was extracted from leaves tissues and 17 microsatellite markers were used to cross-amplification of microsatellite regions.

 

Polymorphism and genetic diversity were evaluated for B. intermedia, B. verbascifolia, B. laxiflora, B. subterranea, B. umbellata, B. linearifolia. from 16 individuals and for B. viminifolia from 14 individuals. Transferred microsatellite markers panels ranged from 11 (64.8%) in B. viminifolia to 6 (35.2%) in B. umbellata. The total number of alleles per locus ranged from 5 (B. linearifolia) to 8 (B. subterranea) alleles. B. umbellata showed lower values of observed and expected heterozygosity (HO = 0.312; HE = 0.436) and B. subterranea presented the highest values (HO = 0.687; HE = 0.778).

A greater number of microsatellite markers should be developed for B. umbellata. The microsatellite marker panels transferred to the species B. intermedia, B. verbascifolia, B. laxiflora, B. subterranea, B. viminifolia and B. linearifolia are very informative, with a high combined probability of exclusion of paternity (Q ≥ 0.976) and the low combined probability of identity (I ≤ 9.91 × 10-6), potentially suitable for future genetic-population studies, supporting strategies for maintaining the genetic diversity and for exploration of Byrsonima species as genetic resources.

 

Xpert Total Histone Extraction Kit

H7200-050 50 ext
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Xpert Total Histone Extraction Kit

H7200-100 100 ext
EUR 610.8

EpiQuik Total Histone H3 Quantification Kit (Colorimetric) 

P-3062-48 48 Assays
EUR 330

EpiQuik Total Histone H3 Quantification Kit (Colorimetric) 

P-3062-96 96 Assays
EUR 599.5

EpiQuik Total Histone H3 Quantification Kit (Fluorometric) 

P-3063-48 48 Assays
EUR 333.3

EpiQuik Total Histone H3 Quantification Kit (Fluorometric) 

P-3063-96 96 Assays
EUR 625.9

EpiQuik Total Histone H4 Quantification Kit (Colorimetric) 

P-3072-48 48 Assays
EUR 328.9

EpiQuik Total Histone H4 Quantification Kit (Colorimetric) 

P-3072-96 96 Assays
EUR 611.6

EpiQuik Total Histone H4 Quantification Kit (Fluorometric) 

P-3073-48 48 Assays
EUR 328.9

EpiQuik Total Histone H4 Quantification Kit (Fluorometric) 

P-3073-96 96 Assays
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EpiQuik Total Histone H3 Quantification Kit (Colorimetric) 

P-3062
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  • 96 Assays

EpiQuik Total Histone H3 Quantification Kit (Fluorometric) 

P-3063
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EpiQuik Total Histone H4 Quantification Kit (Colorimetric) 

P-3072
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  • EUR 611.60
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  • 96 Assays

EpiQuik Total Histone H4 Quantification Kit (Fluorometric) 

P-3073
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  • 96 Assays

EpiQuik Nuclear Extraction Kit 

OP-0002-1 100 Extractions
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EpiQuik Nuclear Extraction Kit 

OP-0002
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EpiQuik Circulating Total Histone H3 Quantification Kit (Colorimetric)

P-3091-48 48 Assays
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EpiQuik Circulating Total Histone H3 Quantification Kit (Colorimetric)

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EpiQuik Circulating Total Histone H3 Quantification Kit (Colorimetric)

P-3091
  • EUR 928.26
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  • EUR 624.80
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Total Protein Extraction Kit

BSP003 50Preps
EUR 130.99

Total Protein Extraction Kit

K3011010 1 kit
EUR 444
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

EpiQuik Whole Cell Extraction Kit 

OP-0003-100 100 Extractions
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EpiQuik Whole Cell Extraction Kit 

OP-0003
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EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Colorimetric) 

P-4030-48 48 Assays
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EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Colorimetric) 

P-4030-96 96 Assays
EUR 613.8

EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Fluorometric) 

P-4031-48 48 Assays
EUR 347.6

EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Fluorometric) 

P-4031-96 96 Assays
EUR 625.9

EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Colorimetric) 

P-4032-48 48 Assays
EUR 347.6

EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Colorimetric) 

P-4032-96 96 Assays
EUR 625.9

EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Fluorometric) 

P-4033-48 48 Assays
EUR 347.6

EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Fluorometric) 

P-4033-96 96 Assays
EUR 625.9

EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Colorimetric) 

P-4030
  • EUR 928.26
  • EUR 343.20
  • EUR 613.80
  • 96 Assays
  • 48 Assays
  • 96 Assays

EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Fluorometric) 

P-4031
  • EUR 945.66
  • EUR 347.60
  • EUR 625.90
  • 96 Assays
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  • 96 Assays

EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Colorimetric) 

P-4032
  • EUR 945.66
  • EUR 347.60
  • EUR 625.90
  • 96 Assays
  • 48 Assays
  • 96 Assays

EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Fluorometric) 

P-4033
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  • EUR 347.60
  • EUR 625.90
  • 96 Assays
  • 48 Assays
  • 96 Assays

Mammalian Total Protein Extraction Kit

20-abx098853
  • EUR 427.20
  • EUR 126.00
  • 100 ml
  • 2 ml

AnaPrep Total RNA Extraction Kit

Z1322015 1 kit (48 extractions) Including all required plastic disposables
EUR 369.6
Description: This kit is developped for our fully automated magnetic bead-based nucleic acid extraction platform which uses preprogrammed protocols and can process up to 12 samples simultaneously. With the AnaPrep 12 Extractor you will have the option to choose and work with a wide range of sample and reagent volumes. This sturdy, realiable and user-friendly machine will save you both time and expenses while ensuring consistently high quality performance and nucleic acids for your downstream applications.

xTractPure Total RNA extraction Kit

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Human Total PSA (t-PSA) ELISA Kit

PRB-5049-TOTAL 96 assays
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Rapid Animal Total RNA Extraction Kit

AT4181 50Preps
EUR 105.67

Rapid Animal Total RNA Extraction Kit

AT4182 250preps
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Total Protein Extraction Kit: TM Buffer

K3011010-1 13 ml
EUR 194.4
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein Extraction Kit: 50X PI

K3011010-2 260 ul
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

EpiQuik Total RNA Isolation Fast Kit

P-9105-050 50 samples
EUR 176

AffiSelect Total Protein Extraction Solution

A0710-015 15X1ml
EUR 170.4

Bacterial Total Protein Extraction Reagent

abx090632-50100assays 50-100 assays
EUR 309.6

Plant Total Protein Extraction Reagent

abx090633-50100assays 50-100 assays
EUR 276

Human Total PSA (t-PSA) ELISA Kit

PRB-5049-TOTAL-5 5 x 96 assays
EUR 2739.6

3-min Bacterial Total Protein Extraction Kit

P512L - Ask for price

EpiQuik Nuclear Extraction Kit II (Nucleic Acid-Free) 

OP-0022-100 100 Extractions
EUR 206.8

EpiQuik Nuclear Extraction Kit II (Nucleic Acid-Free) 

OP-0022
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  • EUR 206.80
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  • 100 Extractions

RNA isolater Total RNA Extraction Reagent

R401-01 100 ml
EUR 159.6

Extract-EZ E1, Total Subcellular Protein Extraction Kit

BSP073 50Preps
EUR 162.31

3-min Total Protein Extraction Kit (Animal cells)

P501 - Ask for price

3-min Total Protein Extraction Kit (Animal cells)

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3-min Total Protein Extraction Kit (Animal cells)

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3-min Total Protein Extraction Kit (Animal Tissues)

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3-min Total Protein Extraction Kit (Animal Tissues)

P502L - Ask for price

3-min Total Protein Extraction Kit (Animal Tissues)

P502S - Ask for price

8-min Plant Tissue Total Protein Extraction Kit

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Total Protein Extraction Kit for Tendons (50 tests)

P5A04 - Ask for price

Total Protein Extraction Kit for Muscles (50 tests)

P5A06 - Ask for price

Total Protein Extraction Kit for Insects (50 tests)

P5A07 - Ask for price

AnaPrep Total RNA Extraction Kit with DNase-Treatment

Z1322017 1 kit (48 extractions) Including all required plastic disposables
EUR 486
Description: This kit is developped for our fully automated magnetic bead-based nucleic acid extraction platform which uses preprogrammed protocols and can process up to 12 samples simultaneously. With the AnaPrep 12 Extractor you will have the option to choose and work with a wide range of sample and reagent volumes. This sturdy, realiable and user-friendly machine will save you both time and expenses while ensuring consistently high quality performance and nucleic acids for your downstream applications.

EpiQuik Methyl-Histone H3K9 ChIP Kit 

P-2006-24 24 Reactions
EUR 442.2

EpiQuik Methyl-Histone H3K9 ChIP Kit 

P-2006-48 48 Reactions
EUR 699.6

EpiQuik Methyl-Histone H3K4 ChIP Kit 

P-2007-24 24 Reactions
EUR 416.9

EpiQuik Methyl-Histone H3K4 ChIP Kit 

P-2007-48 48 Reactions
EUR 686.4

EpiQuik Acetyl-Histone H3 ChIP Kit 

P-2010-24 24 Reactions
EUR 442.2

EpiQuik Acetyl-Histone H3 ChIP Kit 

P-2010-48 48 Reactions
EUR 699.6

EpiQuik Acetyl-Histone H4 ChIP Kit 

P-2011-24 24 Reactions
EUR 442.2

EpiQuik Acetyl-Histone H4 ChIP Kit 

P-2011-48 48 Reactions
EUR 699.6

EpiQuik Methyl-Histone H3K27 ChIP Kit 

P-2015-24 24 Reactions
EUR 490.6

EpiQuik Methyl-Histone H3K27 ChIP Kit 

P-2015-48 48 Reactions
EUR 710.6

EpiQuik Methyl-Histone H3K9 ChIP Kit 

P-2006
  • EUR 1050.06
  • EUR 442.20
  • EUR 699.60
  • 48 Reactions
  • 24 Reactions
  • 48 Reactions

EpiQuik Methyl-Histone H3K4 ChIP Kit 

P-2007
  • EUR 1032.66
  • EUR 416.90
  • EUR 686.40
  • 48 Reactions
  • 24 Reactions
  • 48 Reactions

EpiQuik Acetyl-Histone H3 ChIP Kit 

P-2010
  • EUR 1050.06
  • EUR 442.20
  • EUR 699.60
  • 48 Reactions
  • 24 Reactions
  • 48 Reactions

EpiQuik Acetyl-Histone H4 ChIP Kit 

P-2011
  • EUR 1050.06
  • EUR 442.20
  • EUR 699.60
  • 48 Reactions
  • 24 Reactions
  • 48 Reactions

EpiQuik Methyl-Histone H3K27 ChIP Kit 

P-2015
  • EUR 1067.46
  • EUR 490.60
  • EUR 710.60
  • 48 Reactions
  • 24 Reactions
  • 48 Reactions

Histone/DNA Binding Protein Extraction Kit (cells)

P514-25 - Ask for price

Histone/DNA Binding Protein Extraction Kit (cells)

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Histone/DNA Binding Protein Extraction Kit (cells)

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Total Protein Extraction Kit for Skin Tissue (50 tests)

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Total Protein Extraction Kit for Bone Tissue (50 tests)

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Total Protein Extraction Kit for Blood Vessels (50 tests)

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Total RNA Extraction Kit (Proteinase K & DNase I included)

TR05 5 preps Ask for price

Total RNA Extraction Kit (Proteinase K & DNase I included)

TR100 100 preps
EUR 378

Total RNA Extraction Kit (Proteinase K & DNase I included)

TR25 25 preps
EUR 176.4

Total RNA Extraction Kit (Proteinase K & DNase I included)

TR50 50 preps
EUR 253.2

EZ-10 Spin Column Viral Total RNA Extraction Kit

VT82112 50Preps
EUR 244.52

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