Due to the rapid expansion of microbial taxonomy, an accurate identification of common mushrooms in industry and agriculture such as trichoderma species are difficult. In this study, we introduce the online multi-line identification system (fog) for automated three hundred and forty-nine trichoderm species based on a set of three DNA barcodes. The fog is based on the reference databases of validated sequences of three typical phylogenetic markers collected from public databases.
The databases consist of four hundred and sixteen complex sequences of the internal transcrips of the nuclear RNA 1 and 2 (STI), five hundred and eighty-three gene sequence fragments encoding the translation of the elongation factor 1- Alpha (TEF1) and five hundred thirty -fold sequence fragments of the polymerase RNA 2 (RPB2) gene. Thanks to mists, information from different DNA barcodes can be combined and the identification of trichoderma species can be obtained according to the search for integrated parametric sequence (BLASTN) in the manner of a classifier. decision tree. In the verification process, MDIS provided correct identification for forty-four Trichoderma spp. Based on DNA barcodes consisting of TEF1 and RPB2 markers. Thus, the haze can be used to obtain an automated species identification as well as to retrieve sequences required for manual identification using phylogenetic analysis.
Importance the Trichoderma genus is important for the human type, with a Wide range of applications in industry, agriculture and bioremediation. Thus, a rapid and precise identification of Trichoderma species is paramount because it is usually the first step in Trichoderma-based search. However, it frequently becomes a limitation, especially for researchers who lack taxonomic knowledge of fungi. In addition, the number of trichoderma studies increased, a growing number of unidentified sequences have been stored in public databases, which has made the identification of more ambiguous species.
In this study, we provide an easy-to-use tool for the automated identification of species (fog), a list of trichoderma species and corresponding reference DNA barcode sequences. As a result, this study will facilitate research on biodiversity and trichoderma applications.
Modern modeling methods for fermentation processes.
For effective monitoring and control of the fermentation process, a real-time measurement of the important variables is required. These variables are very difficult to measure in real time due to constraints such as variation in variable time, non-linearity, strong coupling and complex mechanism of the fermentation process. The construction of flexible sensors with exceptional performance and robustness has become a basic problem in industrial procedures. In this article, a complete review of existing data pre-processing approaches, variable selection methods, modeling methods and data data detection optimization techniques (black box) was performed.
Data-oriented methods used for data detection modeling such as the support vector support machine, the more and more square support vector machine, the neuronal network, deep learning, blurred logic , Probabilistic latent variable models are examined in detail. Optimization techniques used for the estimation of the model parameters such as the particle swarm optimization algorithm, the optimization of ant colonies, the artificial bee colony, the cuckoo search algorithm And the genetic algorithm, are also discussed.
A complete analysis of various software detection models is presented in tabular form that highlights the important methods used in the field of fermentation. More than 70 research publications on variable estimation software detection modeling methods have been examined and listed for fast reference.
Examination of interior errors of metabolism causing progressive intellectual and neurological deterioration (PIN).
Progressive intellectual and neurological deterioration (PIN) is a rare but severe childhood disorder characterized by a loss of intellectual or development capabilities and requires rapid diagnosis to ensure rapid treatment to avoid potential irreversible neurological damage. Inherent errors of metabolism (IEMs) constitute a group of more than 1,000 monogenic conditions in which the deficiency of a biochemical route is intrinsic to disease pathophysiology, resulting in an accumulation of toxic metabolites and / or the shortage of blocks. Blocks. energy and construction for cells. Many IEMs are likely to deal with the potential to improve the results. With this review of the literature, we aim to create an overview of IEM with Pind in children, to help clinicians accelerate the diagnostic process.
We did a PubMed search on IEMS with Pind in people aged 0 to 18 years. We applied strict selection criteria and subsequently derived information on genes coding, pathways, clinical and biochemical signs and IEMBase diagnostic tests and other sources. The PubMed search resulted in a total of 2,152 articles and a review of references added yet 19 articles. After applying our selection criteria, a total of 85 IEMs with PIN remained, of which 57 IEM were reported in multiple unbound cases and 28 in single families. For 44 IEMS (52%), a diagnosis can be achieved through metabolic blood and urine detection tests generally accessible; The rest requires enzymatic and / or genetic tests. Treatment Targeting Underlying physiopathology is available for IEMM (41%). All treatment strategies are declared for the stabilization of the deterioration and a subset enhanced the control and / or the nineworthiness of the seizure.
We present the first full overview of IEMP with the PIN and we provide a structured approach to diagnosis and overview of trafficking. Clearly IEM is the most important group of genetic pine conditions and benefits from the advantage of detectable biomarkers as well as the possibility of treatment.
Thus, the clinician should keep IEM at the point of a child’s diagnostic walk with PIN. With the continued discovery of new IEMs, extensive phenotypes and new treatment strategies, continuous updates of this work will be needed.