The alkylimidazolium tetrafluoroborate ionic liquids (ILs) ([C_nmim][BF₄] n = 2, 4, 6, 8, 10) and anionic surfactant sodium dodecyl sulfate (SDS) had been combined collectively to offer environment friendly mediums for chromogenic catalysis of tetramethylbenzidine (TMB) with horseradish peroxidase (HRP) throughout the presence of H₂O₂. The chromogenic effectivity, kinetic conduct, and the potential influencing mechanism for the chromogenic catalysis of HRP-H₂O₂-TMB had been talked about intimately. Therein, the roles of ionic liquids (ILs) had been highlighted by the combination of experiments and theoretical calculations. The SDS/[C₄mim][BF₄] combination displayed superiority in chromogenic catalysis by bettering every the substrate solubility and product stability to the utmost extent potential.

Furthermore, SDS/[C₄mim][BF₄] combination confirmed uniqueness for TMB in bettering the chromogenic effectivity in distinction with totally different chromogenic substrates of HRP. Impressed by the atmosphere pleasant chromogenic system, an enhanced enzyme-linked immunosorbent assay approach for the detection of human immunoglobulin G was established and the fragile colorimetric strategies for the detection of H₂O₂ and glucose had been extra developed through the use of SDS/[C₄mim][BF₄] combination as a result of the medium of chromogenic catalysis of HRP-H₂O₂-TMB. This distinctive chromogenic system is endowed with multitude of potential functions in natural strategies.