QuickDetect Hydroxylysine (Human) ELISA Package deal
( Catalog # E4714-100 ; 96 assays ; Storage at 4ºC )
05/19
I. Introduction:
Hydroxylysine (Hyl) is an amino acid which arises from a post-translational hydroxy modification of lysine. It is most typically known as a
a part of collagen. QuickDetect™ Hydroxylysine (Human) ELISA Package deal makes use of a double-antibody sandwich enzyme-linked immunosorbent one-step course of assay to assay the extent of hydroxylysine in samples.
Commonplace, check out sample and HRP-labeled hydroxylysine (antibodies had been added to enzyme wells which are Pre-coated with hydroxylysine antibody, then carry out incubation and wash to remove the uncombined enzyme.
Upon together with Chromogen Reply A and B, the color of the liquid will turn into blue, and the response with the acid will set off the color to alter into yellow. The depth of color and the main focus of the hydroxylysine sample are positively correlated.
II. Features:
• This ELISA bundle is used for in vitro quantitative dedication of Human Hydroxylysine
• Assay fluctuate: 9.3ng/ml- 300ng/ml
• Accuracy: Commonplace linear regression correlation coefficient R with the anticipated price of the main focus, higher than or equal to
0.9900.
III. Sample Sort:
• Plasma
• Cell and tissue custom supernatants
• Serum
• Totally different natural fluids
• Tissue and cell lysates
Abstract
The alkylimidazolium tetrafluoroborate ionic liquids (ILs) ([Cnmim][BF4] n = 2, 4, 6, 8, 10) and anionic surfactant sodium dodecyl sulfate (SDS) had been combined collectively to offer environment friendly mediums for chromogenic catalysis of tetramethylbenzidine (TMB) with horseradish peroxidase (HRP) throughout the presence of H2O2. The chromogenic effectivity, kinetic conduct, and the potential influencing mechanism for the chromogenic catalysis of HRP-H2O2-TMB had been talked about intimately. Therein, the roles of ionic liquids (ILs) had been highlighted by the combination of experiments and theoretical calculations. The SDS/[C4mim][BF4] combination displayed superiority in chromogenic catalysis by bettering every the substrate solubility and product stability to the utmost extent potential.
Furthermore, SDS/[C4mim][BF4] combination confirmed uniqueness for TMB in bettering the chromogenic effectivity in distinction with totally different chromogenic substrates of HRP. Impressed by the atmosphere pleasant chromogenic system, an enhanced enzyme-linked immunosorbent assay approach for the detection of human immunoglobulin G was established and the fragile colorimetric strategies for the detection of H2O2 and glucose had been extra developed through the use of SDS/[C4mim][BF4] combination as a result of the medium of chromogenic catalysis of HRP-H2O2-TMB. This distinctive chromogenic system is endowed with multitude of potential functions in natural strategies.

chromogen answer b
Introduction
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Description: A polyclonal antibody for detection of Beta III Tubulin from Human, Mouse, Rat. This Beta III Tubulin antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthetic peptide |
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ABM40165-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A monoclonal antibody for detection of Beta III tubulin from Human, Mouse, Rat. This Beta III tubulin antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide |
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ABM40165-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A monoclonal antibody for detection of Beta III tubulin from Human, Mouse, Rat. This Beta III tubulin antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide |
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