Genetic variations in autoimmune genes and VKH disease

stjosephs-hospital

Purpose: Vogt-Koyanagi-Harada (VKH) disease is a rare autoimmune disease. The autoimmune response in VKH disease is against the melanin-producing cells; therefore, in affected individuals melanocyte-containing organs manifest disease symptoms including eyes, ears, skin and nervous system. VKH is a multifactorial disease, and the precise cause of the VKH disease is unknown. Studies have suggested that both environmental and genetic factors are responsible for the VKH disease. In this review, the authors have collected all the available literature on the genetics of VKH to their knowledge and discussed the role of genetic variants in causing VKH disease.

Methods: An extensive literature search was performed in order to review all the published studies regarding VKH clinical phenotyping and genetic variants in VKH disease. Medline, PubMed, Cochrane library, and Scopus was searched using combination of keywords.

Results: It was found that variants in HLA genes, IL-12b, TNFSF4, and miR-20-5p genes are significantly associated with VKH; however, variants in genes ATG10, TNIP1 and CLEC16A did not achieve significant genome-wide association threshold. Moreover, polymorphisms in TNIP1 and CLEC16A play a protective role against VKH.

Conclusion: The authors conclude that increased sample size and a more homogeneous VKH patient population may reveal a significant association of variants in ATG10, TNIP1 and CLEC16A genes with VKH disease.

Expression changes of cytotoxicity and apoptosis genes in HTLV-1-associated myelopathy/tropical spastic paraparesis patients from the perspective of system virology

 

Although human T-cell lymphotropic virus type-1 (HTLV-1) was the first retrovirus among human pathogens to be identified, insufficient information on the pathogenesis of HTLV-1 infection means that no precise mechanism has yet been provided for HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP).

Based on previous studies, it was found that apoptosis and inflammation stimulation were among the most important mechanisms underlying HAM/TSP. The present study provides an in-silico analysis of the microarray data related to HAM/TSP patients. Expression changes of the genes responsible for cytotoxicity and apoptosis processes of HAM/TSP patients and asymptomatic carriers were investigated.

Expression of the genes involved in cytotoxicity and apoptosis in HAM/TSP patients was decreased; hence, a model was proposed indicating that the spread of immune responses in HAM/TSP may be due to expression of HTLV-1 virulence factors and the resistance of HTLV-1-infected cells to apoptosis.

 

Characterization of PcLEA14, a Group 5 Late Embryogenesis Abundant Protein Gene from Pear ( Pyruscommunis) 

Fruit trees need to overcome harsh winter climates to ensure perennially; therefore, they are strongly influenced by environmental stress. In the present study, we focused on the pear homolog PcLEA14 belonging to the unique 5C late embryogenesis abundant (LEA) protein group for which information is limited on fruit trees. PcLEA14 was confirmed to belong to this protein group using phylogenetic tree analysis, and its expression was induced by low-temperature stress. The seasonal fluctuation in its expression was considered to be related to its role in enduring overwinter temperatures, which is particularly important in perennially.

Moreover, the function of PcLEA14 in low-temperature stress tolerance was revealed in transgenic Arabidopsis. Subsequently, the pear homolog of dehydration-responsive element-binding protein/C-repeat binding factor1 (DREB1), which is an important transcription factor in low-temperature stress tolerance and is uncharacterized in pear, was analyzed after bioinformatics analysis revealed the presence of DREB cis-regulatory elements in PcLEA14 and the dormancy-related gene, both of which are also expressed during low temperatures.

Among the five PcDREBsPcDREB1A and PcDREB1C exhibited similar expression patterns to PcLEA14 whereas the other PcDREBs were not expressed in winter, suggesting their different physiological roles. Our findings suggest that the low-temperature tolerance mechanism in overwintering trees is associated with group 5C LEA proteins and DREB1.

 

A role for microRNAs in the epigenetic control of sexually dimorphic gene expression in the human placenta

Aim: The contribution of miRNAs as epigenetic regulators of sexually dimorphic gene expression in the placenta is unknown.

Materials & methods: 382 placentas from the extremely low gestational age newborns (ELGAN) cohort were evaluated for expression levels of 37,268 mRNAs and 2,102 miRNAs using genome-wide RNA-sequencing. Differential expression analysis was used to identify differences in the expression based on the sex of the fetus.

Results: Sexually dimorphic expression was observed for 128 mRNAs and 59 miRNAs. A set of 25 miRNA master regulators was identified that likely contribute to the sexual dimorphic mRNA expression.

Conclusion: These data highlight sex-dependent miRNA and mRNA patterning in the placenta and provide insight into a potential mechanism for observed sex differences in outcomes.

 

Oxidative Stress and Analysis of Selected SNPs of ACHE (rs 2571598), BCHE (rs 3495), CAT (rs 7943316), SIRT1 (rs 10823108), GSTP1 (rs 1695), and Gene GSTM1, GSTT1 in Chronic Organophosphates Exposed Groups from Cameroon and Pakistan 

The detrimental effects of organophosphates (OPs) on human health are thought to be of systemic, i.e., irreversible inhibition of acetylcholinesterase (AChE) at nerve synapses. However, several studies have shown that AChE inhibition alone cannot explain all the toxicological manifestations in prolonged exposure to OPs.

The present study aimed to assess the status of antioxidants malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) (reduced), catalase, and ferric reducing antioxidant power (FRAP) in chronic OP-exposed groups from Cameroon and Pakistan.

Molecular analysis of genetic polymorphisms (SNPs) of glutathione transferases (GSTM1, GSTP1, GSTT1), catalase gene (CAT, rs7943316), sirtuin 1 gene (SIRT1, rs10823108), acetylcholinesterase gene (ACHE, rs2571598), and butyrylcholinesterase gene (BCHE, rs3495) were screened in the OP-exposed individuals to find the possible causative association with oxidative stress and toxicity.

Cholinesterase and antioxidant activities were measured by colorimetric methods using a spectrophotometer. Salting-out method was employed for DNA extraction from blood followed by restriction fragment length polymorphism (RFLP) for molecular analysis. Cholinergic enzymes were significantly decreased in OP-exposed groups. Catalase and SOD were decreased and MDA and FRAP were increased in OP-exposed groups compared to unexposed groups in both groups. GSH was decreased only in Pakistani OPs-exposed group.

Molecular analysis of ACHE, BCHE, Catalase, GSTP1, and GSTM1 SNPs revealed a tentative association with their phenotypic expression that is level of antioxidant and cholinergic enzymes. The study concludes that chronic OPs exposure induces oxidative stress which is associated with the related SNP polymorphism. The toxicogenetics of understudied SNPs were examined for the first time to our understanding. The findings may lead to a newer area of investigation on OPs induced health issues and toxicogenetics.

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stjosephs-hospital

 

Efficient Non-Viral Gene Modification of Mesenchymal Stromal Cells from Umbilical Cord Wharton’s Jelly with Polyethylenimine

 

  • Mesenchymal stromal cells (MSC) derived from human umbilical cord Wharton’s jelly (WJ) have a wide therapeutic potential in cell therapy and tissue engineering because of their multipotential capacity, which can be reinforced through gene therapy in order to modulate specific responses. However, reported methodologies to transfect WJ-MSC using cationic polymers are scarce. Here, WJ-MSC were transfected using 25 kDa branched- polyethylenimine (PEI) and a DNA plasmid encoding GFP.
  • PEI/plasmid complexes were characterized to establish the best transfection efficiencies with lowest toxicity. Expression of MSC-related cell surface markers was evaluated. Likewise, immunomodulatory activity and multipotential capacity of transfected WJ-MSC were assessed by CD2/CD3/CD28-activated peripheral blood mononuclear cells (PBMC) cocultures and osteogenic and adipogenic differentiation assays, respectively.
  • An association between cell number, PEI and DNA content, and transfection efficiency was observed. The highest transfection efficiency (15.3 ± 8.6%) at the lowest toxicity was achieved using 2 ng/μL DNA and 3.6 ng/μL PEI with 45,000 WJ-MSC in a 24-well plate format (200 μL).
  • Under these conditions, there was no significant difference between the expression of MSC-identity markers, inhibitory effect on CD3+ T lymphocytes proliferation and osteogenic/adipogenic differentiation ability of transfected WJ-MSC, as compared with non-transfected cells. These results suggest that the functional properties of WJ-MSC were not altered after optimized transfection with PEI.

 

Mouse FYN Oncogene Related To SRC/FGR/YES (FYN) ELISA Kit

DLR-FYN-Mu-48T 48T
EUR 527
Description: A sandwich quantitative ELISA assay kit for detection of Mouse FYN Oncogene Related To SRC/FGR/YES (FYN) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse FYN Oncogene Related To SRC/FGR/YES (FYN) ELISA Kit

DLR-FYN-Mu-96T 96T
EUR 688
Description: A sandwich quantitative ELISA assay kit for detection of Mouse FYN Oncogene Related To SRC/FGR/YES (FYN) in samples from tissue homogenates, cell lysates or other biological fluids.

Human FYN Oncogene Related To SRC/FGR/YES (FYN) ELISA Kit

RDR-FYN-Hu-48Tests 48 Tests
EUR 544

Human FYN Oncogene Related To SRC/FGR/YES (FYN) ELISA Kit

RDR-FYN-Hu-96Tests 96 Tests
EUR 756

Mouse FYN Oncogene Related To SRC/FGR/YES (FYN) ELISA Kit

RDR-FYN-Mu-48Tests 48 Tests
EUR 557

Mouse FYN Oncogene Related To SRC/FGR/YES (FYN) ELISA Kit

RDR-FYN-Mu-96Tests 96 Tests
EUR 774

Human FYN Oncogene Related To SRC/FGR/YES (FYN) ELISA Kit

RD-FYN-Hu-48Tests 48 Tests
EUR 521

Human FYN Oncogene Related To SRC/FGR/YES (FYN) ELISA Kit

RD-FYN-Hu-96Tests 96 Tests
EUR 723

Mouse FYN Oncogene Related To SRC/FGR/YES (FYN) ELISA Kit

RD-FYN-Mu-48Tests 48 Tests
EUR 533

Mouse FYN Oncogene Related To SRC/FGR/YES (FYN) ELISA Kit

RD-FYN-Mu-96Tests 96 Tests
EUR 740

FYN Antibody

1-CSB-PA143385
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against FYN. Recognizes FYN from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000

FYN Antibody

1-CSB-PA225018
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against FYN. Recognizes FYN from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000

FYN Antibody

1-CSB-PA009101LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against FYN. Recognizes FYN from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

FYN Antibody

1-CSB-PA008520
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against FYN. Recognizes FYN from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/10000

FYN Antibody

1-CSB-PA002595
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against FYN. Recognizes FYN from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/5000

FYN Antibody

43186-100ul 100ul
EUR 252

Fyn Antibody

AF6102 200ul
EUR 304
Description: Fyn Antibody detects endogenous levels of total Fyn.

Fyn Antibody

AF7697 200ul
EUR 376
Description: Fyn Antibody detects endogenous levels of Fyn.

FYN Antibody

BF0309 200ul
EUR 376
Description: FYN antibody detects endogenous levels of total FYN.

Fyn Antibody

ABF6102 100 ug
EUR 438

FYN antibody

10R-10608 100 ug
EUR 349
Description: Mouse monoclonal FYN antibody

FYN antibody

10R-11001 100 ug
EUR 349
Description: Mouse monoclonal FYN antibody

FYN antibody

70R-5772 50 ug
EUR 467
Description: Rabbit polyclonal FYN antibody raised against the N terminal of FYN

FYN antibody

70R-5867 50 ug
EUR 467
Description: Rabbit polyclonal FYN antibody raised against the middle region of FYN

FYN antibody

70R-31289 100 ug
EUR 327
Description: Rabbit polyclonal FYN antibody

FYN antibody

70R-49768 100 ul
EUR 287
Description: Purified Polyclonal FYN antibody

Fyn Polyclonal Antibody

ES5416-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Fyn from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Fyn Polyclonal Antibody

ES5416-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Fyn from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Anti-Fyn Antibody

PB9197 100ug/vial
EUR 294

Fyn Polyclonal Antibody

ES2382-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Fyn from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Fyn Polyclonal Antibody

ES2382-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Fyn from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

FYN (pS21) Antibody

20-abx123287
  • EUR 495.00
  • EUR 704.00
  • EUR 217.00
  • EUR 356.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

FYN (pY416) Antibody

20-abx123288
  • EUR 495.00
  • EUR 704.00
  • EUR 217.00
  • EUR 356.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Fyn Polyclonal Antibody

ABP54417-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of Fyn from Human, Mouse, Rat. This Fyn antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Fyn around the non-phosphorylation site of Y213

Fyn Polyclonal Antibody

ABP54417-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of Fyn from Human, Mouse, Rat. This Fyn antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Fyn around the non-phosphorylation site of Y213

Fyn Polyclonal Antibody

ABP54417-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of Fyn from Human, Mouse, Rat. This Fyn antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Fyn around the non-phosphorylation site of Y213

FYN (pT530) Antibody

20-abx009886
  • EUR 314.00
  • EUR 467.00
  • EUR 203.00
  • 100 ul
  • 200 ul
  • 30 ul

Fyn (pY530) Antibody

abx010767-100ug 100 ug
EUR 439

Fyn Polyclonal Antibody

ABP51383-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of Fyn from Human, Mouse, Rat. This Fyn antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Fyn around the non-phosphorylation site of Y530

Fyn Polyclonal Antibody

ABP51383-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of Fyn from Human, Mouse, Rat. This Fyn antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Fyn around the non-phosphorylation site of Y530

Fyn Polyclonal Antibody

ABP51383-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of Fyn from Human, Mouse, Rat. This Fyn antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Fyn around the non-phosphorylation site of Y530

FYN Polyclonal Antibody

A59178 100 µg
EUR 570.55
Description: The best epigenetics products

Anti-FYN antibody

STJ110903 100 µl
EUR 393
Description: This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.

Anti-FYN antibody

STJ11100097 100 µl
EUR 277
Description: This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.

Anti-Fyn antibody

STJ93170 200 µl
EUR 197
Description: Rabbit polyclonal to Fyn.

Anti-Fyn antibody

STJ93171 200 µl
EUR 197
Description: Fyn is a protein encoded by the FYN gene which is approximately 60,7 kDa. Fyn is localised to the cytoplasm, cell membrane and nucleus. It is involved in RET signalling, GAB1 signalosome, EPH-Ephrin signalling, HIV life cycle and the development Slit-Robo signalling. It is a membrane-associated tyrosine kinase that plays a role in the regulation of cell growth and survival, cell adhesion, integrin-mediated signalling, cytoskeletal remodelling, cell motility, immune response and axon guidance. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Fyn isoform 1 is highly expressed in the brain and isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes. Mutations in the FYN gene may result in Wiskott-Aldrich syndrome. STJ93171 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Fyn protein.

Anti-Fyn antibody

STJ99129 200 µl
EUR 197
Description: Mouse monoclonal to Fyn.

Anti-Fyn antibody

STJ98091 100 µl
EUR 234
Description: Mouse monoclonal to Fyn.

Anti-Fyn antibody

STJ98092 100 µl
EUR 234
Description: Mouse monoclonal to Fyn.

Anti-Fyn antibody

STJ16100691 100 µg
EUR 524

Anti-FYN Antibody

STJ501109 100 µg
EUR 476

Anti-FYN antibody

STJ70057 100 µg
EUR 359

Fyn Polyclonal Antibody

40943-100ul 100ul
EUR 252

Fyn Polyclonal Antibody

40943-50ul 50ul
EUR 187

FYN (pY530) Antibody

abx333497-100ul 100 ul
EUR 467

Monoclonal Fyn Antibody

AMM03159G 0.1ml
EUR 484
Description: A Monoclonal antibody against Human Fyn. The antibodies are raised in Mouse. This antibody is applicable in WB, ICC

FYN Conjugated Antibody

C43186 100ul
EUR 397

Fyn antibody (Tyr530)

70R-37433 100 ug
EUR 349
Description: Rabbit Polyclonal Fyn antibody (Tyr530)

FYN antibody (Tyr530)

70R-31288 100 ug
EUR 327
Description: Rabbit polyclonal FYN antibody (Tyr530)

Fyn/ Rat Fyn ELISA Kit

ELI-09878r 96 Tests
EUR 886

Monoclonal SYN / FYN Antibody (aa7-176, clone FYN-59), Clone: FYN-59

AMM01704G 0.05mg
EUR 484
Description: A Monoclonal antibody against Human SYN / FYN (aa7-176, clone FYN-59). The antibodies are raised in Mouse and are from clone FYN-59. This antibody is applicable in WB and IHC-P, IP

Monoclonal SYN / FYN Antibody (aa7-176, clone FYN-01), Clone: FYN-01

AMM01732G 0.05mg
EUR 484
Description: A Monoclonal antibody against Human SYN / FYN (aa7-176, clone FYN-01). The antibodies are raised in Mouse and are from clone FYN-01. This antibody is applicable in WB and IHC-P, ICC, IP

FYN siRNA

20-abx917352
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

FYN siRNA

20-abx917353
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

FYN siRNA

20-abx902045
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

anti-Fyn

YF-PA11887 50 ul
EUR 363
Description: Mouse polyclonal to Fyn

anti-Fyn

YF-PA11888 100 ug
EUR 403
Description: Rabbit polyclonal to Fyn

anti-Fyn

YF-PA23745 50 ul
EUR 334
Description: Mouse polyclonal to Fyn

anti-Fyn

YF-PA23746 50 ul
EUR 334
Description: Mouse polyclonal to Fyn

FYN Peptide

45-059P 0.1 mg
EUR 338
Description: (NT) FYN Peptide

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