Species Reactivity : Human (Homo sapiens)
UniProt : P33121
Abbreviation : ACSL1
Alternative Names : ACS1; FACL1; FACL2; LACS; LACS1; LACS2; fatty-acid-Coenzyme A ligase; long-chain 1|fatty-acid-Coenzyme A ligase; long-chain 2|lignoceroyl-CoA synthase|long-chain acyl-CoA synthetase 1|long-chain acy
Application : ELISA
Range : 31.25-2000 pg/mL
Sensitivity : 7.8 pg/mL
Intra-AssayCV : ?4.1%
Inter-AssayCV : ?6.6%
Recovery : 0.9
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate ACSL1 in samples. An antibody specific for ACSL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACSL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for ACSL1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACSL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Long-chain-fatty-acid--CoA ligase 1 is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Altho µgh differing in substrate specificity, subcell µLar localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. In melanocytic cells ACSL1 gene expression may be reg µLated by MITF.Activation of long-chain fatty acids for both synthesis of cell µLar lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).