Species Reactivity : Human (Homo sapiens)
UniProt : Q9UBY5
Abbreviation : LPAR3
Alternative Names : EDG7; Edg-7; FLJ98231; GPCR; HOFNH30; LP-A3; LPA3; RP4-678I3; LPA receptor EDG7|OTTHUMP00000011573|calcium-mobilizing lysophosphatidic acid receptor LP-A3|endothelial cell differentiation gene 7|end
Application : ELISA
Range : 0.312-20 ng/mL
Sensitivity : 0.113 ng/mL
Intra-AssayCV : ?5.3%
Inter-AssayCV : ?6.3%
Recovery : 0.81
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate LPAR3 in samples. An antibody specific for LPAR3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPAR3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for LPAR3 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPAR3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : LPA and the structurally related lysolipid mediator sphingosine 1-phosphate (S1P) signal cells thro µgh a set of G protein-coupled receptors known as EDG receptors. Some EDG receptors (e.g., EDG1) are S1P receptors; others (e.g., EDG2) are LPA receptors. EDG7 mediates responses to unsaturated LPA .Sequence analysis predicted that the 353-amino acid EDG7 protein shares approximately 54% and 49% amino acid identity with EDG2 and EDG4, respectively, and less than 40% identity with the S1P-specific receptors (e.g., EDG1). Northern blot analysis detected a 4.3-kb EDG7 transcript in heart, pancreas, prostate, and testis, with lower expression in lung, ovary, and some cancer cell lines. Western blot analysis showed that transfected cells express EDG7 as an approximately 35-kD protein.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).