Species Reactivity : Human (Homo sapiens)
UniProt : P18627
Abbreviation : LAG3
Alternative Names : CD223; lymphocyte-activation protein 3
Application : ELISA
Range : 78.1-5000 pg/mL
Sensitivity : 30 pg/mL
Intra-AssayCV : ?5.9%
Inter-AssayCV : ?10.1%
Recovery : 0.89
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate LAG3 in samples. An antibody specific for LAG3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAG3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for LAG3 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAG3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : LAGE3 belongs to the ESO/LAGE gene family, members of which are clustered together on chromosome Xq28, and have similar exon-intron structures. Unlike the other family members which are normally expressed only in testis and activated in a wide range of human tumors, this gene is ubiquitously expressed in somatic tissues. The latter, combined with the finding that it is highly conserved in mouse and rat, s µggests that the encoded protein is functionally important. An intronless pseudogene with high sequence similarity to this gene is located on chromosome 9. By searching EST databases with genomic sequences obtained from CpG islands located in the Xq28 region, Faranda et al. (1996) identified a gene, which they called ITBA2. The gene is relatively short, contains a 105-amino acid open reading frame, and is ubiquitously expressed.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).