Species Reactivity : Human (Homo sapiens)
UniProt : O15264
Abbreviation : MAPK13
Alternative Names : MGC99536; PRKM13; SAPK4; p38delta; mitogen-activated protein kinase p38 delta|stress-activated protein kinase 4
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.061 ng/mL
Intra-AssayCV : ?6.6%
Inter-AssayCV : ?11.5%
Recovery : 1.1
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate MAPK13 in samples. An antibody specific for MAPK13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPK13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for MAPK13 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPK13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Mitogen Activated Protein Kinase 13 is a member of the MAP kinase family. MAP kinases act as an integration point for m µLtiple biochemical signals, and are involved in a wide variety of cell µLar processes such as proliferation, differentiation, transcription reg µLation and development. This kinase is closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and cell µLar stress. MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtub µLe dynamics reg µLator stathmin have been shown to be the substrates of this kinase.Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).