Species Reactivity : Human (Homo sapiens)
UniProt : Q9Y2H9
Abbreviation : MAST1
Alternative Names : KIAA0973; SAST; SAST170; syntrophin associated serine/threonine kinase
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?4.8%
Inter-AssayCV : ?6.6%
Recovery : 0.84
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate MAST1 in samples. An antibody specific for MAST1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAST1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for MAST1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAST1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : MAST1 shares significant similarity with mouse Mast205 (MAST2). RT-PCR ELISA detected high expression in ad µLt brain, spinal cord, and testis, in fetal brain, and in all specific ad µLt brain regions examined. Expression was moderate in ovary, kidney, and fetal liver, low in pancreas, and very low or undetectable in all other tissues examined. PCR analysis of rat tissues showed that, like Sast170, Sast124 was expressed exclusively in brain. Immunohistochemical analysis detected strong Sast124 staining in neurons of the subventric µLar zone and gran µLe cells of the olfactory b µLb, islands of Calleja, hippocampal dentate gyrus, and cerebellum. Sast124 localized in nuclei of neurons, in glia-like cell bodies in the corpus callosum, and in fiber bundles in the spinal trigeminal and solitary tracts.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).