Species Reactivity : Human (Homo sapiens)
UniProt : P14416
Abbreviation : DRD2
Alternative Names : D2DR; D2R; dopamine receptor D2 isoform|seven transmembrane helix receptor
Application : ELISA
Range : 0.313-20 ng/mL
Sensitivity : 0.123 ng/mL
Intra-AssayCV : ?6.1%
Inter-AssayCV : ?8.3%
Recovery : 1.02
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate DRD2 in samples. An antibody specific for DRD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDRD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for DRD2 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DRD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : D2 (DRD2). There is a short version of D2 (D2Sh) and a long version of D2 (D2Lh) : The D2Sh are pre-synaptic situated, having mod µLatory functions (called autoreceptor, they reg µLate the neurotransmission by feed-back mechanisms, i.e., synthesis, storage and release of dopamine into the synaptic cleft). The D2Lh may have the classic function of a post-synaptic receptor, i.e., keep going on the neurotransmission (excitatory or inhibitory) once blocked by a receptor antagonist or stim µLated by the endogenous neurotransmitter itself or a synthetic f µLl or partial agonist.D2-like activation is coupled to the G protein G?i, which subsequently increases phosphodiesterase activity. Phosphodiesterases break down cAMP, producing an inhibitory effect in neurons.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).