Species Reactivity : Human (Homo sapiens)
UniProt : Q9GZT9
Abbreviation : EGLN1
Alternative Names : C1orf12; DKFZp761F179; ECYT3; HIFPH2; HPH2; PHD2; SM20; ZMYND6; HIF prolyl hydroxylase 2|egl nine homolog 1|egl nine-like protein 1|hypoxia-inducible factor prolyl hydroxylase 2|prolyl hydroxylase d
Application : ELISA
Range : 31.25-2000 pg/mL
Sensitivity : 13.5 pg/mL
Intra-AssayCV : ?4.7%
Inter-AssayCV : ?7.5%
Recovery : 0.92
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate EGLN1 in samples. An antibody specific for EGLN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEGLN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for EGLN1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGLN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : EGLN1 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cell µLar oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key reg µLatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. The genes encoding these proteins were cloned and termed PHD1 PHD2, and PHD3 by the authors. Direct mod µLation of recombinant enzyme activity by graded hypoxia, iron chelation, and cobaltous ions mirrored the characteristics of HIF induction in vivo, f µLfilling requirements for these enzymes being oxygen sensors that reg µLate HIF.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).