Species Reactivity : Human (Homo sapiens)
UniProt : Q9Y485
Abbreviation : DMXL1
Alternative Names : DKFZp779O1239; FLJ44772;
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?4.1%
Inter-AssayCV : ?8.6%
Recovery : 0.99
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate DMXL1 in samples. An antibody specific for DMXL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMXL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for DMXL1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMXL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : By EST database searching for sequences showing homology to Drosophila DmX and by use of primers designed for RT-PCR from human placental mRNA, Kraemer et al. (2000) cloned a nearly f µLl-length homolog, which they designated DMX-like-1. The DMXL1 cDNA encodes a deduced 3,027-amino acid protein with a calc µLated mass of approximately 338 kD. The protein belongs to the superfamily of WD repeat proteins, most of which have reg µLatory functions, and contains at least 28 WD repeat units. DMXL1 shows a high level of evolutionary conservation. By searching EST databases with the complete cDNA sequence as query, Kraemer et al. (2000) found that DMXL1 is expressed in a variety of tissues, including bone, breast, eye, foreskin, heart, parathyroid, small intestine, testis, tonsils, uterus, and whole embryo.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).