Species Reactivity : Human (Homo sapiens)
UniProt : Q6IQ55
Abbreviation : TTBK2
Alternative Names : KIAA0847; SCA11; TTBK; spinocerebellar ataxia 11
Application : ELISA
Range : 0.312-20 ng/mL
Sensitivity : 0.124 ng/mL
Intra-AssayCV : ?5.8%
Inter-AssayCV : ?10.8%
Recovery : 0.89
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TTBK2 in samples. An antibody specific for TTBK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTTBK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TTBK2 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TTBK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : TTBK2 produces a 5.6-kb transcript in which the longest open reading frame is 3,732 nucleotides, encoding a protein of 1244 amino acids. The gene is alternatively spliced, with ubiquitous expression in human ad µLt and fetal tissues. The N-terminus of TTBK2 encodes a serine-threonine-tyrosine kinase domain, and a C-terminal region shows homology to TTBK1. TTBK2 was expressed in all brain regions in human, rat, and mouse tissue. There was partic µLarly high expression in Purkinje cells of the cerebellum, gran µLar cell layer, hippocampus, midbrain, and substantia nigra. Lower expression was seen in the cortex of human, rat, and mouse brains.2 TTBK2 phosphorylation sites in tau (ser208 and ser210) are priming sites for the phosphorylation of tau by GSK-3-beta, which influences tau pathology.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).