Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : MAPK3,MAPK1
GeneID : 5595,5594
Uniprot : P27361/P28482
Specificity : ERK1/2 Antibody detects endogenous levels of total ERK1/2.
Immunogen : A syntheVolumed peptide derived from human ERK1/2, corresponding to a region within C-terminal amino acids.
Storage : PBS, pH 7.4,50% glycerol.
Reactivity : Human,Mouse,Rat,Pig,Zebrafish,Bovine,Horse,Sheep,Dog,Monkey,Fish
Prediction : Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit
Application : WB,IHC,IF/ICC,IP,ELISA(peptide)
Dilution : WB : 1 : 1000-1 : 5000, IHC : 1 : 100-1 : 500, IF/ICC 1 : 200 IP 1 : 200, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2833336
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : AF0155-BP
Des# Blocking Peptide : ERK1/2 Blocking peptide
Blocking Peptide Price : $549 CAD