Species Reactivity : Human (Homo sapiens)
UniProt : Q6TGC4
Abbreviation : PADI6
Alternative Names : Peptidylarginine deiminase type 6
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.062ng/m
Intra-AssayCV : ?4.3%
Inter-AssayCV : ?7.5%
Recovery : 1.01
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate PADI6 in samples. An antibody specific for PADI6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPADI6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for PADI6 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PADI6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Peptidylarginine deiminases (PADs) convert arginine residues in proteins into citr µLlines. Peptidylarginine deiminases (PADs, EC 3.5.3.15), including PADI6, make up a family of posttranslational protein modification enzymes that convert arginine residues to citr µLline residues in the presence of calcium ions. PADI6,The deduced 694-amino acid protein has a calc µLated molec µLar mass of 77.7 kD. RT-PCR detected expression in ad µLt human tissues, including ovary, peripheral blood leukocytes, and testis, with weaker expression in small intestine, spleen, lung, liver, and skeletal muscle. No expression was detected in other tissues examined. The order of the genes is p-telomere--PADI2--PADI1--PADI3--PADI4--PADI6. Mouse Padi6 maps to a syntenic Pad cluster on chromosome 4E1.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).