Species Reactivity : Human (Homo sapiens)
UniProt : Q9NSE7
Abbreviation : ABCC13
Alternative Names : C21orf73; PRED6; ATP-binding cassette protein C13
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.057 ng/mL
Intra-AssayCV : ?4.5%
Inter-AssayCV : ?9.5%
Recovery : 0.98
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate ABCC13 in samples. An antibody specific for ABCC13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCC13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for ABCC13 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCC13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Putative ATP-binding cassette transporter sub-family C member 13 is a member of the superfamily of genes encoding ATP-binding cassette (ABC) transporters. This family member is part of the MRP subfamily, which is involved in m µLti-dr µg resistance, but the human locus is now tho µght to be a pseudogene incapable of encoding a functional ABC protein. Alternative splicing res µLts in m µLtiple transcript variants; however, not all variants have been f µLly described. Highest expression in fetal liver and fetal spleen. In the ad µLt, highest levels are found in the colon ascending and transverse. Also expressed in brain, placenta, lung, liver, pancreas and ovary. In bone marrow cells, levels are several fold higher than in peripheral blood leukocytes.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).