Species Reactivity : Human (Homo sapiens)
UniProt : Q9UKR0
Abbreviation : KLK12
Alternative Names : DKFZp686H1078; KLK-L5; KLKL5; MGC42603; kallikrein 12|kallikrein-like protein 5
Application : ELISA
Range : 12.35-1000 ng/mL
Sensitivity : 7.14 ng/mL
Intra-AssayCV : ?5.1%
Inter-AssayCV : ?9.6%
Recovery : 1
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate KLK12 in samples. An antibody specific for KLK12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for KLK12 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Kallikrein-12 is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene res µLts in three transcript variants encoding different isoforms.In the second mRNA form, the last exon is split into 2 separate exons with an additional intervening intron, producing a deduced 254-amino acid protein with a predicted molec µLar mass of 27.1 kD. In the third mRNA form, the fourth exon is missing, producing a deduced 111-amino acid protein with a molec µLar mass of 12 kD. RT-PCR demonstrated that KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).