Species Reactivity : Human (Homo sapiens)
UniProt : P05231
Abbreviation : IL6
Alternative Names : BSF2; HGF; HSF; IFNB2; IL-6; B cell stim µLatory factor-2|B-cell differentiation factor|CTL differentiation factor|OTTHUMP00000158544|hybridoma growth factor|interleukin 6|interleukin BSF-2
Application : ELISA
Range : 7.8-500 pg/mL
Sensitivity : 3.2 pg/mL
Intra-AssayCV : ?5.1%
Inter-AssayCV : ?9.4%
Recovery : 0.99
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate IL6 in samples. An antibody specific for IL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for IL6 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : IL-6 is a m µLtifunctional protein produced by lymphoid and non-lymphoid cells, and by normal and transformed cells, including T cells, monocyte/macrophages, fibroblasts, hepatocytes, vasc µLar endothelial cells, cardiac myxomas, bladder cell carcinomas, myelomas, astrogliomas and glioblastomas. The production of IL-6 in these various cells is reg µLated, either positively or negatively, by a variety of signals including mitogens, antigenic stim µLation, lipopolysaccharides, IL-1 TNF, PDGF and viruses. On the basis of its various activities, IL-6 has also been called interferon-?2 (IFN-?2), 26 kDa protein, B-cell stim µLatory factor-2 (BSF-2),?hybridoma/plasmacytoma growth factor, hepatocyte stim µLating factor, cytotoxic T-cell differentiation factor, and macrophage-gran µLocyte inducing factor 2A (MGI-2A).
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).