Species Reactivity : Human (Homo sapiens)
UniProt : Q5SZQ8
Abbreviation : TNRC4
Alternative Names : BRUNOL1; CAGH4; CELF3; ERDA4; MGC57297; CAG repeat domain|C µg-BP and ETR-3 like factor 3|expanded repeat domain; CAG/CTG 4
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?5.9%
Inter-AssayCV : ?8.2%
Recovery : 0.84
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TNRC4 in samples. An antibody specific for TNRC4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNRC4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TNRC4 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNRC4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : CELF proteins, including CELF3, bound RNA containing muscle-specific splicing enhancer (MSE) elements and co µLd activate MSE-dependent exon inclusion of cardiac troponin T (TNNT2) minigenes in transfected quail fibroblasts.The deduced 462-amino acid protein contains 2 N-terminal RNA recognition motifs (RRMs), a C-terminal RRM, several potential phosphorylation sites, and a nuclear localization signal near the C terminus. activated inclusion of exon 10 in a tau (MAPT) minigene. Deletion and mutation analyses showed that the activity of TNRC4 on tau exon 10 splicing required RRM2 in TNRC4 and an intronic element in tau pre-mRNA. The polyglutamine sequence of TNRC4 had no effect on tau exon 10 splicing. Tnrc4 -/- mice exhibited decreased inclusion of exon 10 in tau mRNA.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).