Species Reactivity : Human (Homo sapiens)
UniProt : N/A
Abbreviation : Cr
Alternative Names : N/A
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.059 ng/mL
Intra-AssayCV : ?6.1%
Inter-AssayCV : ?11.5%
Recovery : 1
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate Cr in samples. An antibody specific for Cr has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCr present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for Cr is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Cr bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Calretinin is a calcium-binding protein, 29 kDa, member of the family of so-called EF-hand proteins, to which also the S-100 proteins belong. Calretinin is abundantly expressed in neurons. Outside the nervous system, calretinin is found in mesothelial cells, steroid producing cells (adrenal cortical cells, testic µLar Leydig cells, ovarian theca interna cells), testic µLar Sertoli cells, rete testis, ovarian surface epithelium, some neuroendocrine cells, breast glands, eccrine sweat glands, hair follic µLar cells, thymic epithelial cells, endometrial stromal cells, and fat cells. In calretinin positive cells, the protein is generally found in both the cytoplasm and nuclei.Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).