Species Reactivity : Human (Homo sapiens)
UniProt : P03973
Abbreviation : SLPI
Alternative Names : ALK1; ALP; BLPI; HUSI; HUSI-I; MPI; WAP4; WFDC4; WAP four-dis µLfide core domain 4|antileukoproteinase|mucus proteinase inhibitor|secretory leukocyte protease inhibitor (antileukoproteinase)|seminal
Application : ELISA
Range : 62.5-4000 pg/mL
Sensitivity : 27.1 pg/mL
Intra-AssayCV : ?4.3%
Inter-AssayCV : ?7.1%
Recovery : 0.99
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate SLPI in samples. An antibody specific for SLPI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLPI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for SLPI is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLPI bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Antileukoproteinase is secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes; the protein is also tho µght to have broad-spectrum anti-biotic activity.Secretory leukocyte protease inhibitor (SLPI) is a highly cationic single-chain protein with eight intramolec µLar dis µLfide bonds. It is found in large quantities in bronchial, cervical, and nasal mucosa, saliva, and seminal fluids. SLPI inhibits human leukocyte elastase, human cathepsin G, human trypsin, neutrophil elastase, and mast cell chymase.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).