Species Reactivity : Human (Homo sapiens)
UniProt : Q13470
Abbreviation : TNK1
Alternative Names : MGC46193;
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?4.5%
Inter-AssayCV : ?7.6%
Recovery : 0.93
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TNK1 in samples. An antibody specific for TNK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TNK1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : TNK1 is a nonreceptor tyrosine kinase. These kinases, like members of the SRC and JAK families, mediate intracell µLar signaling downstream of receptor activation. TNK1 contains a putative N-terminal phosphotyrosine-binding domain, followed by the tyrosine kinase domain, a putative SH3 domain, and a proline-rich C terminus that contains SH3-binding motifs. The kinase domain has all 12 structural features conserved in tyrosine kinases, including an ATP-binding site. TNK1 shares highest homology with ACK, including 45% amino acid identity over a 413-amino acid span that includes the kinase domain and putative SH3 domain. specific expression of 4 transcripts between 1.0 and 9.5 kb in fetal brain, lung, liver, and kidney.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).