Species Reactivity : Human (Homo sapiens)
UniProt : Q9UEF7
Abbreviation : KL
Alternative Names : N/A
Application : ELISA
Range : 15.6-1000 pg/mL
Sensitivity : 6.6 pg/mL
Intra-AssayCV : ?4.8%
Inter-AssayCV : ?8.4%
Recovery : 0.98
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate KL in samples. An antibody specific for KL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for KL is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Klotho is a type-I membrane protein that is related to ?-glucuronidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. Klotho is a transmembrane protein that, in addition to other effects, provides some control over the sensitivity of the organism to ins µLin and appears to be involved in aging. Its discovery was documented in 1997 by Kuro-o et al. The name of the gene comes from Klotho or Clotho, one of the Moirae, or Fates, in Greek mythology.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).