Species Reactivity : Human (Homo sapiens)
UniProt : P56470
Abbreviation : LGALS4
Alternative Names : GAL4; L36LBP; L-36 lactose-binding protein|galectin 4|galectin-4|lactose-binding lectin 4
Application : ELISA
Range : 62.5-4000 pg/mL
Sensitivity : 26.4 pg/mL
Intra-AssayCV : ?4.6%
Inter-AssayCV : ?7.1%
Recovery : 0.99
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate LGALS4 in samples. An antibody specific for LGALS4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGALS4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for LGALS4 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Galectin-4 belongs to a subfamily of galectins composed of two carbohydrate recognition domains within the same peptide chain. The two domains have all the conserved galectin signature amino acids, but their overall sequences are only approximately 40% identical. Both domains bind lactose with a similar affinity as other galectins, but their respective preferences for other disaccharides, and larger saccharides, are distinctly different. Thus galectin-4 has a property of a natural cross-linker, but in a modified sense since each domain prefers a different subset of ligands. Similarly to other galectins, galectin-4 is syntheVolumed as a cytosolic protein, but can be externalized. During development and in ad µLt normal tissues, galectin-4 is expressed only in the alimentary tract, from the tongue to the large intestine.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).