Species Reactivity : Human (Homo sapiens)
UniProt : Q9NX76
Abbreviation : CMTM6
Alternative Names : CKLFSF6; FLJ20396; PRO2219; chemokine-like factor super family 6|chemokine-like factor superfamily 6
Application : ELISA
Range : 61.7-5000 pg/mL
Sensitivity : 2.9 pg/mL
Intra-AssayCV : ?5.0%
Inter-AssayCV : ?7.8%
Recovery : 0.93
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate CMTM6 in samples. An antibody specific for CMTM6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCMTM6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for CMTM6 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CMTM6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Members of the chemokine-like factor superfamily, including CKLFSF6, share similarity with both the chemokine and the transmembrane 4 superfamilies of signaling molec µLes.By screening databases for sequences that share homology with chemokine-like factor (CKLF), followed by RT-PCR of a prostate cDNA library, Han et al. (2003) cloned CKLFSF6. The deduced 183-amino acid CKLFSF6 protein has 4 transmembrane segments. It shares about 31% sequence identity with CKLFSF4 . Northern blot analysis detected a transcript of 3.88 kb expressed in leukocytes, placenta, and testis. A smaller transcript was also detected in leukocytes. Han et al. (2003) cloned mouse Cklfsf6, which shares about 88.5% sequence similarity with CKLFSF6.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).