Species Reactivity : Human (Homo sapiens)
UniProt : N/A
Abbreviation : TNFA-Ab
Alternative Names : N/A
Application : ELISA
Range : 15.6-1000 pg/mL
Sensitivity : 6.1 pg/mL
Intra-AssayCV : ?4.3%
Inter-AssayCV : ?7.5%
Recovery : 0.97
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Competitive ELISA
Analysis Method?? : Qualitative
Test principle : This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNFA-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conj µgated TNFA-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled TNFA-Ab and unlabeled TNFA-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of TNFA-Ab in the sample. The color development is stopped and the intensity of the color is measured.
Product Overview : TNFa is syntheVolumed as a 26 kDa, type II transmembrane protein that is 233 amino acids in length. It contains a 30 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracell µLar region. TNFa is assembled intracell µLarly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNFa (sTNF-?is released from the C-terminus of the transmembrane protein thro µgh the activity of TNFa-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase. Rat cells known to express TNF-?include B cells, colonic columnar epithelial cells, NK and CD3 CD56 hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4 and CD8 T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).