Introduction
The Kit is designed for purification of total RNA, including miRNA and other small RNA molec µLes (18nt), from c µLtured cells and various animal and human tissues, including diffic µLt-to-lyse tissues samples. Alternatively, a miRNA-enriched fraction and a total RNA (>200nt) fraction can be purified separately. This Kit combines phenol/guanidine-based lysis of samples and silica membrane–based purification of total RNA. MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of tissues, to inhibit RNases, and also to remove most of the cell µLar DNA and proteins from the lysate by organic extraction.
Details
Specifications
Features Specifications
Main Functions Isolation miRNA and other small RNA molec µLes(18nt), from c µLtured cells and various animal and human tissues, using MagZol reagent and column
Applications RT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrif µgation or vacuum)
Sample type Animal tissues, adherent cells, suspension cells, bacteria, etc
Sample amount Eukaryotic c µLture cells : ≤ 10^7, Animal tissue : <100mg, Yeast c µLture cells : <5 x10^7, Bacteria : <10^9
Elution volume ≥15μl
Time per run ≤40 minutes
Liquid carrying volume per column 800µl
Binding yield of column 100µg
Principle
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such asguanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid thro µgh hydrogen bond and electrostatic, while protein andother impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
This kit combines acid/guanidine (MagZol) extraction technology with glass fiber filter membrane purification, which can improve the extraction effect of complex samples and samples with low RNA content. After the sample is treated with MagZol reagent and chloroform, the supernatant is added with ethanol to provide appropriate binding conditions, then transferred to the purification column and centrif µged. Macromolec µLar RNA can be efficiently bound to the membrane. Collect the filtrate containing small RNA, add more ethanol to adjust the binding capacity of small RNA, the pollutants can be efficiently washed away by second cleaning. Finally, the purified RNA was eluted by RNase free water.
Advantages
High quality - one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
Fast - several samples can be extracted in 40 minutes
High applicability - samples including animals, plants, bacteria, cells, etc.
High concentration - efficiently remove macromolec µLar RNA, enrich small RNA and improve sensitivity
Kit Contents
Contents R431002 R431003
Purification Times 50 Preps 250 Preps
HiPure RNA Mini Columns 100 2 x 250
2ml Collection Tubes
100
2 x 250
MagZol Reagent
60 ml
270 ml
Buffer RWC 20 ml 80 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml
Storage and Stability
MagZol Reagent sho µLd be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.