Mitsuhashi and Maramorosch Insect Medium without Sodium bicarbonate

Without: Sodium bicarbonate Mitsuhashi and Maramorosch Insect Medium is specially used for the growth and propogation of mosquito cell lines especially Aedes aegypticus. It was originally developed to grow cells derived from Leafhopper. Subsequently, it has been used to culture cells derived from a number of insect species. IM002 is Mitsuhashi amd Maramorosch Insect Medium . It needs to be supplemented with 5-20 % fetal bovine serum. Lactalbumin hydrolysate serves as a source of free amino acid, whereas extract serves as a source of vitamins. When supplemented with fetal bovine serum, this medium is most commonly used to culture cells derived from a number of mosquitoes. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.