Species Reactivity : Hamster (Mesocricetus; Cricet µLus)
UniProt : N/A
Abbreviation : LXA4
Alternative Names : N/A
Application : ELISA
Range : 493.8-40000 pg/mL
Sensitivity : 175.4 pg/mL
Intra-AssayCV : ?5.8%
Inter-AssayCV : ?8.6%
Recovery : 1
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate LXA4 in samples. An antibody specific for LXA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLXA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for LXA4 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LXA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Lipoxins are a series of anti-inflammatory mediators. Lipoxins are short lived endogenously produced nonclassic eicosanoids whose appearance in inflammation signals the resolution of inflammation. They are abbreviated as LX, an acronym for lipoxygenase (LO) interaction products. At present two lipoxins have been identified; lipoxin A4 () and lipoxin B4 (LXB4).Lipoxins, as well as certain peptides, are high affinity ligands for the lipoxin A4 receptor (LXA4R), which was first identified based on sequence homology as the formyl peptide receptor like receptor (FPRL1). Lipoxin signaling thro µgh the LXA4R inhibits chemotaxis, transmigration, superoxide generation and NF-kappa B activation. Similarly to the leukotrienes, LXA4 will form the cysteinyl-lipoxins LXC4, LXD4 and LXE4. At subnanomolar concentrations, LXA4 and LXB4 inhibit leukotriene-stim µLated interactions of human neutrophils and endothelial cells.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).