Species Reactivity : Canine (Canis familiaris; Dog)
UniProt : B9VTT2
Abbreviation : DLC1
Alternative Names : ARHGAP7; FLJ21120; HP; STARD12; p122-RhoGAP; Rho-GTPase-activating protein 7|StAR-related lipid transfer (START) domain containing 12|deleted in liver cancer 1 variant 2
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?4.2%
Inter-AssayCV : ?9.3%
Recovery : 0.98
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate DLC1 in samples. An antibody specific for DLC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for DLC1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Deleted in Liver Cancer 1 is deleted in the primary tumor of hepatocell µLar carcinoma. It maps to 8p22-p21.3, a region frequently deleted in solid tumors. It is s µggested that this gene is a candidate tumor suppressor gene for human liver cancer, as well as for prostate, lung, colorectal, and breast cancers.DLC1 is frequently inactivated in human hepatocell µLar carcinoma, as well as some nasopharyngeal, lung, breast, prostate, kidney, colon, uterine, ovarian, and gastric cancers. The DLC1 protein contains four major functional domains : an N-terminal sterile ? motif (SAM), a serine-rich (SR) region, a Rho-GAP domain, and a C-terminal steroidogenic acute reg µLatory protein related lipid-transfer (START) domain. DLC1 is localized to focal adhesions located at the periphery of cells.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).