Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : O00602
Abbreviation : FCN1
Alternative Names : FCNM; ficolin (collagen/fibrinogen domain-containing) 1|ficolin 1
Application : ELISA
Range : 1.56-100 ng/mL
Sensitivity : 0.78 ng/mL
Intra-AssayCV : ?6.4%
Inter-AssayCV : ?9.2%
Recovery : 1.01
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate FCN1 in samples. An antibody specific for FCN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFCN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for FCN1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FCN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. The collagen-like and the fibrinogen-like domains are also found separately in other proteins such as complement protein C1q, C-type lectins known as collectins, and tenascins. However, all these proteins recognize different targets, and are functionally distinct.Ficolin 1 encoded by FCN1 is predominantly expressed in the peripheral blood leukocytes, and has been post µLated to function as a plasma protein with elastin-binding activity.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).