Species Reactivity : Human (Homo sapiens)
UniProt : Q9Y243
Abbreviation : AKT3
Alternative Names : DKFZp434N0250; PKB-GAMMA; PKBG; PRKBG; RAC-PK-gamma; RAC-gamma; STK-2; AKT3 kinase|OTTHUMP00000037911|OTTHUMP00000037912|RAC-gamma serine/threonine protein kinase|protein kinase B gamma|serine threo
Application : ELISA
Range : 1.56-100 ng/mL
Sensitivity : 0.60 ng/mL
Intra-AssayCV : ?4.6%
Inter-AssayCV : ?7.3%
Recovery : 0.94
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate AKT3 in samples. An antibody specific for AKT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAKT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for AKT3 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AKT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be reg µLators of cell signaling in response to ins µLin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stim µLated by platelet-derived growth factor (PDGF), ins µLin, and ins µLin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.Mice lacking Akt3 have a normal glucose metabolism (no diabetes), have approximately normal body weight, but have a 25% reduction in brain mass. Incidentally, Akt3 is highly expressed in the brain.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).