Species Reactivity : Human (Homo sapiens)
UniProt : O60635
Abbreviation : TSPAN1
Alternative Names : NET1; TM4C; TM4SF; OTTHUMP00000008900|tetraspan 1
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.074 ng/mL
Intra-AssayCV : ?4.7%
Inter-AssayCV : ?8.3%
Recovery : 1.06
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TSPAN1 in samples. An antibody specific for TSPAN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTSPAN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TSPAN1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSPAN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : TSPAN1 belongs to the large family of tetraspanins, so named because they contain 4 transmembrane domains. Tetraspanins are frequently expressed at the cell surface in association with each other and with other molec µLes, such as integrins, and they function to reg µLate cell adhesion, migration, proliferation, and differentiation. The deduced 242-amino acid protein contains 4 transmembrane domains and other features characteristic of tetraspanins, including conservation of charged residues in or near the transmembrane domains, similar to ion channels, and of 4 cysteines in the second extracell µLar domain. EST database analysis s µggested that TSPAN2 is expressed in colon, endometrium, pancreas, normal and neoplastic prostate, and pregnant uterus.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).