Species Reactivity : Human (Homo sapiens)
UniProt : Q96KP6
Abbreviation : TNIP3
Alternative Names : ABIN-3; FLJ21162; LIND; ABIN-3 beta|Listeria induced|TNFAIP3-interacting protein 3 beta|TNIP3 beta
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?5.2%
Inter-AssayCV : ?8.1%
Recovery : 0.95
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TNIP3 in samples. An antibody specific for TNIP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNIP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TNIP3 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNIP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Using differential display RT-PCR, followed by 5-prime and 3-prime RACE, Staege et al. (2001) isolated a cDNA encoding TNIP3, which they called LIND, that was upreg µLated in monocytes infected with Listeria monocytogenes. The predicted 325-amino acid protein contains 3 coiled-coil domains and is most likely localized to the nucleus. The authors identified potential splice sites that wo µLd res µLt in variants with different C termini. Northern blot analysis detected 1.7- and 2.4-kb transcripts in Listeria-infected monocytes, but not in uninfected controls. RT-PCR analysis showed highest expression in lung, lymph node, thymus, and fetal liver, with lower levels in brain, spleen, leukocytes, and tonsils. Northern blot analysis of m µLtiple tissues showed no expression.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).