Species Reactivity : Human (Homo sapiens)
UniProt : N/A
Abbreviation : RT3
Alternative Names : N/A
Application : ELISA
Range : 74.07-6000 pg/mL
Sensitivity : 27.55 pg/mL
Intra-AssayCV : ?5.8%
Inter-AssayCV : ?9.3%
Recovery : 1.01
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate RT3 in samples. An antibody specific for RT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for RT3 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Reverse triiodothyronine is a molec µLe which is an isomer of triiodothyronine (T3). It is derived from thyroxine (T4) thro µgh the action of deiodinase.rT3, unlike T3, does not stim µLate thyroid hormone receptors. However, rT3 nonetheless binds to these receptors, thereby blocking the action of T3. Under stress conditions, the adrenal glands produce excess amounts of cortisol. Cortisol inhibits the conversion of T4 to T3, thus shunting T4 conversion from T3 towards rT3. Consequently, there is a widespread shutdown in T3 binding across the body. This condition is termed Reverse T3 Dominance. It res µLts in reduced body temperature, which slows the action of many enzymes, leading to a clinical syndrome, M µLtiple Enzyme Dysfunction, which produces the effects seen in hypothyroidism. Effects include fatigue, headache, migraine, PMS, irritability, fluid retention, anxiety and panic.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).