Species Reactivity : Human (Homo sapiens)
UniProt : Q96RD7
Abbreviation : PANX1
Alternative Names : MGC21309; MRS1; PX1; UNQ2529; innexin
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.064 ng/mL
Intra-AssayCV : ?4.1%
Inter-AssayCV : ?7.3%
Recovery : 1.05
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate PANX1 in samples. An antibody specific for PANX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPANX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for PANX1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PANX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Pannexin 1 belongs to the innexin family. Innexin family members are the structural components of gap junctions. This protein and pannexin 2 are abundantly expressed in central nerve system (CNS) and are coexpressed in various neuronal pop µLations. Studies in Xenopus oocytes s µggest that this protein alone and in combination with pannexin 2 may form cell type-specific gap junctions with distinct properties. The deduced 48-kD protein contains 4 transmembrane domains and cytoplasmic N and C termini. Northern blot analysis detected Panx1 expression in rat eye, thyroid, prostate, kidney, liver, and central nervous system. In situ hybridization showed Pnx1 expression in several brain regions, including cortex, striatum, olfactory b µLb, hippocampus, thalamus, and cerebellum.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).