Species Reactivity : Human (Homo sapiens)
UniProt : P41247
Abbreviation : PNPLA4
Alternative Names : DXS1283E; GS2; IPLA2-ETA; GS2
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?5.1%
Inter-AssayCV : ?9.6%
Recovery : 0.99
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate PNPLA4 in samples. An antibody specific for PNPLA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPNPLA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for PNPLA4 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PNPLA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : PNPLA4 encodes a member of the patatin-like family of phospholipases. The encoded enzyme has both triacylglycerol lipase and transacylase activities and may be involved in adipocyte triglyceride homeostasis. Alternate splicing res µLts in m µLtiple transcript variants. A pseudogene of this gene is found on chromosome Y.DNA sequencing of a GS2 cDNA clone demonstrated an open reading frame for a basic protein of 253 amino acid residues and an isoelectric point of 9.8. A polymorphic CT dinucleotide repeat was found in the 3-prime untranslated region. The GS2 gene was expressed in all human tissues examined, including heart, brain, placenta, lung, liver, muscle, kidney, pancreas, and spleen. Several GS2 transcripts, ranging in Volume from 1.1 to 5.8 kb, were found among different tissues, s µggesting tissue-specific processing of the GS2 transcript.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).