Species Reactivity : Human (Homo sapiens)
UniProt : P52797
Abbreviation : EFNA3
Alternative Names : EFL2; EPLG3; Ehk1-L; LERK3; eph-related receptor tyrosine kinase ligand 3|ephrin A3|ligand of eph-related kinase 3
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.051 ng/mL
Intra-AssayCV : ?7.1%
Inter-AssayCV : ?9.4%
Recovery : 1.02
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate EFNA3 in samples. An antibody specific for EFNA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEFNA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for EFNA3 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFNA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Ephrin-A3 is a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin. Expressed in brain, skeletal muscle, spleen, thymus, prostate, testis, ovary, small intestine, and peripheral blood leukocytes.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).