Species Reactivity : Human (Homo sapiens)
UniProt : N/A
Abbreviation : EBv-IgG
Alternative Names : N/A
Application : ELISA
Range : Detection antibody
Sensitivity : Request Information
Intra-AssayCV : ?4.6%
Inter-AssayCV : ?9.4%
Recovery : 0.98
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate EBv-IgG in samples. An antibody specific for EBv-IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEBv-IgG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for EBv-IgG is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EBv-IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : The Epstein-Barr virus (EBV), also called human herpesvirus 4 (HHV-4), is one of eight known viruses in the herpes family, and is one of the most common viruses in humans. It is best known as the cause of infectious mononucleosis (gland µLar fever). It is also associated with partic µLar forms of cancer, such as Hodgkin's lymphoma, Burkitt's lymphoma, gastric cancer, nasopharyngeal carcinoma, and conditions associated with human immunodeficiency virus (HIV), such as hairy leukoplakia and central nervous system lymphomas. There is evidence that infection with EBV is associated with a higher risk of certain autoimmune diseases, especially dermatomyositis, systemic lupus erythematosus, rheumatoid arthritis, Sjögren's syndrome, and m µLtiple sclerosis. Some 200,000 cancer cases per year are tho µght to be attributable to EBV.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).