Species Reactivity : Human (Homo sapiens)
UniProt : N/A
Abbreviation : BAT3
Alternative Names : DAQB-195H10.3; BAG-6; BAG6; D6S52E; G3; HLA-B associated transcript-3|large proline-rich protein BAT3|scythe HLA-B associated transcript 3 (BAT3)
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?7.2%
Inter-AssayCV : ?9.6%
Recovery : 1.01
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate BAT3 in samples. An antibody specific for BAT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBAT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for BAT3 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BAT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : BAT3 contains an N-terminal ubiquitin-like domain, and both BAT2 and BAT3 are rich in proline and include short tracts of polyproline, polyglycine, and charged amino acids.The presence of additional genes was s µggested by a large cluster of CpG islands. With cosmid probes, 5 distinct transcripts, including BAT3, were detected in RNA samples from a variety of cell lines, and the corresponding cDNA clones were isolated.BAT3 formed a complex with p300 (EP300), and an increased amount of BAT3 enhanced recruitment of p53 to p300 and facilitated subsequent p53 acetylation. In contrast, Bat3-depleted cells showed reduced p53-p300 complex formation and decreased p53 acetylation. Thymocytes from Bat3-deficient mice exhibited reduced p53-mediated induction of Puma and p21 and were resistant to DNA damage-induced apoptosis in vivo.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).