BeaverBeads ™ Magrose NHS is spherical beads modified with NHS groups capable of forming stable peptide bonds with proteins and other molec µLes with primary amine groups for affinity purification or assay detection of antibodies, Antigens and other biomolec µLes. Compared with the traditional carboxyl group, amino beads, the surface containing NHS group of beads without EDC / NHS or glutaraldehyde in advance to activate, simply by the primary amino acid containing the ligand dissolved in the coupling buffer , The protein solution is mixed with Magarose NHS beads at room temperature for 1 ~ 2h. The biological ligand can be covalently coupled to the magnetic beads. It has the advantages of simple operation, mild coupling condition and quick and efficient coupling of biological ligands. We also offer the Magnetic bead version of this kit. The difference between BeaverBeads ™ Mag NHS and BeaverBeads ™ Magrose NHS is that Mag NHS is a polymer-based solid microsphere with a particle Volume of 2 μm and is mainly used for analytical areas (eg in vitro diagnostics, microbiological testing, immunoprecipitation, immunization Precipitation, etc.); and Magrose NHS is a natural hydrophilic polymer agarose as the substrate of porous microspheres, particle Volume of 30 ~ 100μm, which has good biocompatibility, a larger surface area and biological Based coupling, mainly used for separation and purification of the field (such as protein separation and purification, antibody isolation and purification).
specification : 10 mg/mL
packaging : 1 mL, 10 mg/mL, 2 μm
Simple, without activation, can be directly covalently coupled with the biological ligand;
Efficient, bio-ligand coupling efficiency of up to 90% or more, much higher than the carboxyl beads, while the ligand with a higher binding capacity;
Fast, 1 ~ 2h to complete the bio-ligand coupling;
Mild, room temperature or 4 ℃ coupling, coupling system pH 5 ~ 9;
Stable, the formation of a stable amide bond, to prevent the ligand off.
Good biocompatibility, reduced nonspecific adsorption.
Applicable scope
It is suitable for covalent coupling of biomolec µLes such as protein, antibody, enzyme, polypeptide and nucleic acid containing primary amino group.
Application direction
In vitro diagnosis
Immunoassay;
Cell sorting;
Immunoprecipitation / co - precipitation;
Protein / antibody isolation and purification.