Volume : 100 µL
Clone Number :
Aliases : IMD22 antibody; LCK antibody; Lck p56 antibody; LCK proto-oncogene; Src family tyrosine kinase antibody; LCK_HUMAN antibody; Leukocyte C-terminal Src kinase antibody; LSK antibody; Lymphocyte cell specific protein tyrosine kinase antibody; Lymphocyte cell-specific protein-tyrosine kinase antibody; Lymphocyte specific protein tyrosine kinase antibody; Membrane associated protein tyrosine kinase antibody; Oncogene lck antibody; P56 LCK antibody; p56(LSTRA) protein tyrosine kinase antibody; p56-LCK antibody; p56lck antibody; pp58 lck antibody; pp58lck antibody; Protein YT16 antibody; Proto oncogene tyrosine protein kinase LCK antibody; Proto-oncogene Lck antibody; Protooncogene tyrosine protein kinase LCK antibody; T cell specific protein tyrosine kinase antibody; T cell-specific protein-tyrosine kinase antibody; T lymphocyte specific protein tyrosine kinase p56lck antibody; Tyrosine-protein kinase Lck antibody; YT 16 antibody; YT16 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P06239
Immunogen : Peptide sequence around aa.392-396(N-E-Y-T-A) derived from Human Lck.
Raised in : Rabbit
Species Reactivity : Human, Mouse
Tested Applications : ELISA, WB; WB : 1 : 500-1 : 1000
Background : Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molec µLes, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stim µLated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molec µLes are recruited, µLtimately leading to lymphokine production. LCK also contributes to signaling by other receptor molec µLes. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor res µLts in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the reg µLation of maturation events that are governed by both pre-TCR and mature a beta TCR. Phosphorylates other substrates including RUNX3, the microtub µLe-associated protein MAPT, RHOH or TYROBP.
Goh Y.M., Cinghu S., Hong E.T., Lee Y.S., Kim J.H., Jang J.W., Li Y.H., Chi X.Z., Lee K.S., Wee H., Ito Y., Oh B.C., Bae S.C.J. Biol. Chem. 285 : 10122-10129(2010)
Wang H., Zeng X., Fan Z., Lim B.Cell. Signal. 23 : 249-258(2011)
Scales T.M., Derkinderen P., Leung K.Y., Byers H.L., Ward M.A., Price C., Bird I.N., Perera T., Kellie S., Williamson R., Anderton B.H., Reynolds C.H.Mol. Neurodegener. 6 : 12-12(2011)
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conj µgates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Conj µgate : Non-conj µgated
Buffer : Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : LCK
Research Areas : Immunology;Signal transduction