Species Reactivity : Human (Homo sapiens)
UniProt : Q9Y286
Abbreviation : SIGLEC7
Alternative Names : AIRM1; CD328; CDw328; D-siglec; QA79; SIGLEC-7; p75; p75/AIRM1; adhesion inhibitory receptor molec µLe 1; siglec-7|sialic acid binding immunoglob µLin-like lectin 7
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.058 ng/mL
Intra-AssayCV : ?5.3%
Inter-AssayCV : ?7.5%
Recovery : 0.97
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate SIGLEC7 in samples. An antibody specific for SIGLEC7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIGLEC7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for SIGLEC7 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : The highest amino acid sequence similarity found was with the myeloid-specific CD33 molec µLe , and similar to other sialoadhesin molec µLes, AIRM1 appears to mediate sialic acid-dependent ligand recognition. By analyzing genomic DNA samples derived from a panel of human/hamster somatic cell hybrids, Falco et al. (1999) mapped the SIGLEC7 gene to chromosome 19. They noted that other members of the sialoadhesin family map to chromosome 19, including CD33, SIGLEC6, SIGLEC5, myelin-associated glycoprotein (MAG), and CD22 . Therefore, SIGLEC7 is probably located on 19q13.3, where these other family members have been localized. Human tissues show strong expression of Siglec-7 mRNA in spleen, peripheral blood leukocytes, and liver.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).