Species Reactivity : Human (Homo sapiens)
UniProt : Q8IWL8
Abbreviation : STH
Alternative Names : MAPTIT; MGC163191; MGC163193; microtub µLe-associated protein tau (MAPT) intronic transcript
Application : ELISA
Range : 0.312-20 ng/mL
Sensitivity : 0.103 ng/mL
Intra-AssayCV : ?6.1%
Inter-AssayCV : ?10.4%
Recovery : 1.01
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate STH in samples. An antibody specific for STH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for STH is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : During the examination of ESTs in the human tau locus, Conrad et al. (2002) identified a gene they designated saitohin, or STH, in honor of the late Dr. Tsuanao Saitoh and his laboratory. The STH gene encodes a predicted 128-amino acid protein.highest expression of STH was in placenta, muscle, fetal brain, and ad µLt brain, with lower expression in heart, kidney, stomach, testis, and adrenal gland. In the central nervous system, highest expression was in temporal lobe, hypothalamus, med µLla, and spinal cord, with lower expression in other brain regions. There was significant overlap with the general tissue and CNS expression of tau, altho µgh there were some differences. The res µLts indicated that STH is not under the reg µLation of the tau promoter, but may share some regional reg µLatory elements with the tau gene.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).